UV and visible spectrophotometric method for the determination of leucovorin

Muhammad Khalid Bhatti; Muhammad Munawar Hayat; Hafiz Muhammad Nauman Shabbir; Syed Nabil Raza Rizvi; Aumbreen Akram; Faiz Ul Hassan Nasim; Muhammad Ashraf; Bushra Hussain; Irshad Ahmad

UV and visible spectrophotometric method for the determination of leucovorin

Muhammad Khalid Bhatti, Muhammad Munawar Hayat, Hafiz Muhammad Nauman Shabbir, Syed Nabil Raza Rizvi, Aumbreen Akram, Faiz Ul Hassan Nasim, Muhammad Ashraf^*, Bushra Hussain, Irshad Ahmad

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Two simple and reproducible UV-Visible spectrophotometric methods were developed for the estimation of leucovorin in pure and injectable dosage forms. The visible method is based on the reaction of leucovorin with Folin-Ciocalteu Reagent (FCR) in the presence of sodium hydroxide which forms a bluish green coloured complex. This mixture was heated for 10 minutes at 70-900C. The resultant complex after suitable dilution with water exhibited maximum absorption at 724 nm. UV spectrophotometric method was developed and used in the direct estimation of leucovorin in pure and injectable dosage forms. In this method, leucovorin in phosphate buffer (pH 7.7) was mixed thoroughly and measurements were taken at 280 nm. Both these methods are simple, low cost and easy to handle for the quantitative determination of leucovorin in injectable pharmaceutical preparations.

Original language	English
Pages (from-to)	1601-1604
Number of pages	4
Journal	Journal of the Chemical Society of Pakistan
Volume	34
Issue number	6
State	Published - Dec 2012
Externally published	Yes

Keywords

Folin-ciocalteu reagent
Injectable dosage forms
Leucovorin
Spectrophotometry

ASJC Scopus subject areas

General Chemistry

Cite this

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title = "UV and visible spectrophotometric method for the determination of leucovorin",

abstract = "Two simple and reproducible UV-Visible spectrophotometric methods were developed for the estimation of leucovorin in pure and injectable dosage forms. The visible method is based on the reaction of leucovorin with Folin-Ciocalteu Reagent (FCR) in the presence of sodium hydroxide which forms a bluish green coloured complex. This mixture was heated for 10 minutes at 70-900C. The resultant complex after suitable dilution with water exhibited maximum absorption at 724 nm. UV spectrophotometric method was developed and used in the direct estimation of leucovorin in pure and injectable dosage forms. In this method, leucovorin in phosphate buffer (pH 7.7) was mixed thoroughly and measurements were taken at 280 nm. Both these methods are simple, low cost and easy to handle for the quantitative determination of leucovorin in injectable pharmaceutical preparations.",

keywords = "Folin-ciocalteu reagent, Injectable dosage forms, Leucovorin, Spectrophotometry",

author = "Bhatti, {Muhammad Khalid} and Hayat, {Muhammad Munawar} and Shabbir, {Hafiz Muhammad Nauman} and Rizvi, {Syed Nabil Raza} and Aumbreen Akram and Nasim, {Faiz Ul Hassan} and Muhammad Ashraf and Bushra Hussain and Irshad Ahmad",

year = "2012",

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language = "English",

volume = "34",

pages = "1601--1604",

journal = "Journal of the Chemical Society of Pakistan",

issn = "0253-5106",

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TY - JOUR

T1 - UV and visible spectrophotometric method for the determination of leucovorin

AU - Bhatti, Muhammad Khalid

AU - Hayat, Muhammad Munawar

AU - Shabbir, Hafiz Muhammad Nauman

AU - Rizvi, Syed Nabil Raza

AU - Akram, Aumbreen

AU - Nasim, Faiz Ul Hassan

AU - Ashraf, Muhammad

AU - Hussain, Bushra

AU - Ahmad, Irshad

PY - 2012/12

Y1 - 2012/12

N2 - Two simple and reproducible UV-Visible spectrophotometric methods were developed for the estimation of leucovorin in pure and injectable dosage forms. The visible method is based on the reaction of leucovorin with Folin-Ciocalteu Reagent (FCR) in the presence of sodium hydroxide which forms a bluish green coloured complex. This mixture was heated for 10 minutes at 70-900C. The resultant complex after suitable dilution with water exhibited maximum absorption at 724 nm. UV spectrophotometric method was developed and used in the direct estimation of leucovorin in pure and injectable dosage forms. In this method, leucovorin in phosphate buffer (pH 7.7) was mixed thoroughly and measurements were taken at 280 nm. Both these methods are simple, low cost and easy to handle for the quantitative determination of leucovorin in injectable pharmaceutical preparations.

AB - Two simple and reproducible UV-Visible spectrophotometric methods were developed for the estimation of leucovorin in pure and injectable dosage forms. The visible method is based on the reaction of leucovorin with Folin-Ciocalteu Reagent (FCR) in the presence of sodium hydroxide which forms a bluish green coloured complex. This mixture was heated for 10 minutes at 70-900C. The resultant complex after suitable dilution with water exhibited maximum absorption at 724 nm. UV spectrophotometric method was developed and used in the direct estimation of leucovorin in pure and injectable dosage forms. In this method, leucovorin in phosphate buffer (pH 7.7) was mixed thoroughly and measurements were taken at 280 nm. Both these methods are simple, low cost and easy to handle for the quantitative determination of leucovorin in injectable pharmaceutical preparations.

KW - Folin-ciocalteu reagent

KW - Injectable dosage forms

KW - Leucovorin

KW - Spectrophotometry

UR - http://www.scopus.com/inward/record.url?scp=84874468410&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84874468410

SN - 0253-5106

VL - 34

SP - 1601

EP - 1604

JO - Journal of the Chemical Society of Pakistan

JF - Journal of the Chemical Society of Pakistan

IS - 6

ER -

UV and visible spectrophotometric method for the determination of leucovorin

Abstract

Keywords

ASJC Scopus subject areas

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