The effect of DNA probe distribution on the reliability of label-free biosensors

M. W. Shinwari, M. F. Shinwari, M. J. Deen*, P. R. Selvaganapathy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

As the design of label-free DNA biosensors matures, and their sizes reduced to enhance their sensitivity, not much has been researched about the variations in the received signal with the positioning of the probes on the sensitive surface. We approach this issue computationally in this paper. By adopting the finite-element model on a three-dimensional biological field-effect transistor (BioFET) slice, and running Monte-Carlo simulations on the positions of the DNA molecules, we extract the expected variations in the signal. Then, we show that signal-to-noise (SNR) ratio can be low enough to hinder the functionality of the device, placing a limitation on how low the sensitivity of a sensor of a certain size can be.

Original languageEnglish
Pages (from-to)441-447
Number of pages7
JournalSensors and Actuators B: Chemical
Volume160
Issue number1
DOIs
StatePublished - 15 Dec 2011
Externally publishedYes

Keywords

  • Biosensor
  • DNA
  • Finite-element
  • Lab-on-chip
  • Label-free
  • Simulation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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