Synthetic reactive polyelectrolytes for cell encapsulation

In this work we studied the synthesis and characterization of reactive polyelectrolytes that are capable of forming covalent cross-links, and that can be used in cell encapsulation. In the first part we outline the synthesis and characterization of these polyelectrolytes, and their use to form covalently cross-linked outer shells around calcium alginate microcapsules. The second part presents the formation of covalently cross-linked networks within the cores of calcium alginate microcapsules. The encapsulation processes, capsule morphology, mechanical strength, permeability and biocompatibility are discussed in some detail. The resulting capsules showed improved mechanical strength yet remain cyto-compatible. This approach to cell-encapsulation may be useful for cell immuno-isolation in therapeutic cell transplants.