Spectrophotometric method for the determination of Gemifloxacin mesylate in pure and tablet dosage form

Syed Saeed Ul Hassan; Uzma Hayat; Imran Tariq; Irshad Ahmad; Muhammad Munawar Hayat; Muhammad Uzair; Muhammad Tayyab Ansari

Spectrophotometric method for the determination of Gemifloxacin mesylate in pure and tablet dosage form

Syed Saeed Ul Hassan^*
, Uzma Hayat
, Imran Tariq
, Irshad Ahmad
, Muhammad Munawar Hayat
, Muhammad Uzair
, Muhammad Tayyab Ansari

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A spectrophotometric method for the determination of Gemifloxacin mesylate (GFX) is developed and validated according to ICH guidelines. GFX is a fluoroquinolone that is used in the treatment of pneumonia. The analysis of the pure drug was carried out at its λ_max270 nm. The method was linear from 0.5-5μg/mL, r²0.999 and equation is 0.102-0.000. The % RSD for inter-day (0.969%) and intra-day (0.714%) assuring a good precision and accuracy was close to 100%. Limit of detection and Limit of quantification were 0.197 and 0.599μg/mL, respectively. The validation results and statistical data demonstrate that the method is accurate, sensitive, cost effective and reproducible and has an importance in quality assurance of GFX analysis. The developed method was proved suitable for analysis of GFX in the pure and tablet dosage forms without interference of excepients.

Original language	English
Pages (from-to)	1171-1174
Number of pages	4
Journal	Pakistan Journal of Pharmaceutical Sciences
Volume	27
Issue number	5
State	Published - 1 Sep 2014
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014, Pakistan Journal of Pharmaceutical Sciences. All rights reserved.

Keywords

Gemifloxacin mesylate
Spectrophotometer
Tablet dosage form
Validation

ASJC Scopus subject areas

Pharmaceutical Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{cf396f547723451990236bfeaf9dfa23,

title = "Spectrophotometric method for the determination of Gemifloxacin mesylate in pure and tablet dosage form",

abstract = "A spectrophotometric method for the determination of Gemifloxacin mesylate (GFX) is developed and validated according to ICH guidelines. GFX is a fluoroquinolone that is used in the treatment of pneumonia. The analysis of the pure drug was carried out at its λmax270 nm. The method was linear from 0.5-5μg/mL, r20.999 and equation is 0.102-0.000. The \% RSD for inter-day (0.969\%) and intra-day (0.714\%) assuring a good precision and accuracy was close to 100\%. Limit of detection and Limit of quantification were 0.197 and 0.599μg/mL, respectively. The validation results and statistical data demonstrate that the method is accurate, sensitive, cost effective and reproducible and has an importance in quality assurance of GFX analysis. The developed method was proved suitable for analysis of GFX in the pure and tablet dosage forms without interference of excepients.",

keywords = "Gemifloxacin mesylate, Spectrophotometer, Tablet dosage form, Validation",

author = "\{Saeed Ul Hassan\}, Syed and Uzma Hayat and Imran Tariq and Irshad Ahmad and Hayat, \{Muhammad Munawar\} and Muhammad Uzair and Ansari, \{Muhammad Tayyab\}",

year = "2014",

month = sep,

day = "1",

language = "English",

volume = "27",

pages = "1171--1174",

journal = "Pakistan Journal of Pharmaceutical Sciences",

issn = "1011-601X",

publisher = "Pakistan Journal of Pharmaceutical Sciences",

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TY - JOUR

T1 - Spectrophotometric method for the determination of Gemifloxacin mesylate in pure and tablet dosage form

AU - Saeed Ul Hassan, Syed

AU - Hayat, Uzma

AU - Tariq, Imran

AU - Ahmad, Irshad

AU - Hayat, Muhammad Munawar

AU - Uzair, Muhammad

AU - Ansari, Muhammad Tayyab

PY - 2014/9/1

Y1 - 2014/9/1

N2 - A spectrophotometric method for the determination of Gemifloxacin mesylate (GFX) is developed and validated according to ICH guidelines. GFX is a fluoroquinolone that is used in the treatment of pneumonia. The analysis of the pure drug was carried out at its λmax270 nm. The method was linear from 0.5-5μg/mL, r20.999 and equation is 0.102-0.000. The % RSD for inter-day (0.969%) and intra-day (0.714%) assuring a good precision and accuracy was close to 100%. Limit of detection and Limit of quantification were 0.197 and 0.599μg/mL, respectively. The validation results and statistical data demonstrate that the method is accurate, sensitive, cost effective and reproducible and has an importance in quality assurance of GFX analysis. The developed method was proved suitable for analysis of GFX in the pure and tablet dosage forms without interference of excepients.

AB - A spectrophotometric method for the determination of Gemifloxacin mesylate (GFX) is developed and validated according to ICH guidelines. GFX is a fluoroquinolone that is used in the treatment of pneumonia. The analysis of the pure drug was carried out at its λmax270 nm. The method was linear from 0.5-5μg/mL, r20.999 and equation is 0.102-0.000. The % RSD for inter-day (0.969%) and intra-day (0.714%) assuring a good precision and accuracy was close to 100%. Limit of detection and Limit of quantification were 0.197 and 0.599μg/mL, respectively. The validation results and statistical data demonstrate that the method is accurate, sensitive, cost effective and reproducible and has an importance in quality assurance of GFX analysis. The developed method was proved suitable for analysis of GFX in the pure and tablet dosage forms without interference of excepients.

KW - Gemifloxacin mesylate

KW - Spectrophotometer

KW - Tablet dosage form

KW - Validation

UR - https://www.scopus.com/pages/publications/84907833981

M3 - Article

C2 - 25176374

AN - SCOPUS:84907833981

SN - 1011-601X

VL - 27

SP - 1171

EP - 1174

JO - Pakistan Journal of Pharmaceutical Sciences

JF - Pakistan Journal of Pharmaceutical Sciences

IS - 5

ER -

Spectrophotometric method for the determination of Gemifloxacin mesylate in pure and tablet dosage form

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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