Solid-phase purification of gene synthesis products using magnetic beads

Mo Chao Huang; Mo Huang Li; Jackie Y. Ying

doi:10.1117/12.810439

Solid-phase purification of gene synthesis products using magnetic beads

Mo Chao Huang, Mo Huang Li, Jackie Y. Ying

Research output: Contribution to journal › Conference article › peer-review

Abstract

Herein we report three novel methods which utilize chemical conjugated magnetic beads to purify synthetic gene from its synthesis solution and prepare the synthesized gene in suitable buffer for downstream applications. Silica-coated magnetic beads are applied for non-specific DNA purification to remove short oligonucleotides and monomers. Streptavidin conjugated magnetic beads and (dT)₂₅ Oligo immobilized magnetic beads are introduced for specific DNA extraction. The performances of these methods are investigated and compared using gel electrophoresis. The optimal conditions for enhancing the extraction efficiency are discussed. In addition, the approach to integrate these solid-phase purification methods into microfluidic devices is presented.

Original language	English
Article number	72690A
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	7269
DOIs	https://doi.org/10.1117/12.810439
State	Published - 2008
Externally published	Yes
Event	Micro- and Nanotechnology: Materials, Processes, Packaging, and Systems IV - Melbourne, VIC, Australia Duration: 10 Dec 2008 → 12 Dec 2008

Keywords

DNA extraction
Gene synthesis
Magnetic beads
PCR purification
Solid-phase purification

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.810439

Cite this

@article{523cb8cd10354d9abd850d26a5e1a08f,

title = "Solid-phase purification of gene synthesis products using magnetic beads",

abstract = "Herein we report three novel methods which utilize chemical conjugated magnetic beads to purify synthetic gene from its synthesis solution and prepare the synthesized gene in suitable buffer for downstream applications. Silica-coated magnetic beads are applied for non-specific DNA purification to remove short oligonucleotides and monomers. Streptavidin conjugated magnetic beads and (dT)25 Oligo immobilized magnetic beads are introduced for specific DNA extraction. The performances of these methods are investigated and compared using gel electrophoresis. The optimal conditions for enhancing the extraction efficiency are discussed. In addition, the approach to integrate these solid-phase purification methods into microfluidic devices is presented.",

keywords = "DNA extraction, Gene synthesis, Magnetic beads, PCR purification, Solid-phase purification",

author = "Huang, \{Mo Chao\} and Li, \{Mo Huang\} and Ying, \{Jackie Y.\}",

year = "2008",

doi = "10.1117/12.810439",

language = "English",

volume = "7269",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Micro- and Nanotechnology: Materials, Processes, Packaging, and Systems IV ; Conference date: 10-12-2008 Through 12-12-2008",

}

TY - JOUR

T1 - Solid-phase purification of gene synthesis products using magnetic beads

AU - Huang, Mo Chao

AU - Li, Mo Huang

AU - Ying, Jackie Y.

PY - 2008

Y1 - 2008

N2 - Herein we report three novel methods which utilize chemical conjugated magnetic beads to purify synthetic gene from its synthesis solution and prepare the synthesized gene in suitable buffer for downstream applications. Silica-coated magnetic beads are applied for non-specific DNA purification to remove short oligonucleotides and monomers. Streptavidin conjugated magnetic beads and (dT)25 Oligo immobilized magnetic beads are introduced for specific DNA extraction. The performances of these methods are investigated and compared using gel electrophoresis. The optimal conditions for enhancing the extraction efficiency are discussed. In addition, the approach to integrate these solid-phase purification methods into microfluidic devices is presented.

AB - Herein we report three novel methods which utilize chemical conjugated magnetic beads to purify synthetic gene from its synthesis solution and prepare the synthesized gene in suitable buffer for downstream applications. Silica-coated magnetic beads are applied for non-specific DNA purification to remove short oligonucleotides and monomers. Streptavidin conjugated magnetic beads and (dT)25 Oligo immobilized magnetic beads are introduced for specific DNA extraction. The performances of these methods are investigated and compared using gel electrophoresis. The optimal conditions for enhancing the extraction efficiency are discussed. In addition, the approach to integrate these solid-phase purification methods into microfluidic devices is presented.

KW - DNA extraction

KW - Gene synthesis

KW - Magnetic beads

KW - PCR purification

KW - Solid-phase purification

UR - https://www.scopus.com/pages/publications/65349125841

U2 - 10.1117/12.810439

DO - 10.1117/12.810439

M3 - Conference article

AN - SCOPUS:65349125841

SN - 0277-786X

VL - 7269

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

M1 - 72690A

T2 - Micro- and Nanotechnology: Materials, Processes, Packaging, and Systems IV

Y2 - 10 December 2008 through 12 December 2008

ER -

Solid-phase purification of gene synthesis products using magnetic beads

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this