Abstract
A highly sensitive H-point standard addition method (HPSAM) was investigated for identification of dopamine and ascorbic acid in some synthetic and pharmaceutical samples in an aqueous medium at pH 9. 2 using a universal buffer. The most suitable wavelengths for dopamine and ascorbic acid detection are 260:271 nm and 248:270 nm respectively. Recovery values are between 99. 0-101 %. Also, the effect of most common interferents was studied. Detection ranges for dopamine and ascorbic acid are 2·10-6-5·10-5 M and 6·10-6-3·10-5 M respectively with an RSD range between 1. 3 and 2. 0 %. The method was used to determine both reagents in real and synthesized samples.
| Original language | English |
|---|---|
| Pages (from-to) | 424-430 |
| Number of pages | 7 |
| Journal | Journal of Applied Spectroscopy |
| Volume | 79 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jul 2012 |
| Externally published | Yes |
Keywords
- HPSAM
- UV
- ascorbic acid
- dopamine
- pharmaceutical samples
- urine samples
ASJC Scopus subject areas
- Condensed Matter Physics
- Spectroscopy