Silicatein-mediated incorporation of titanium into spicules from the demosponge Suberites domuncula

Filipe Natalio; Enrico Mugnaioli; Matthias Wiens; Xiaohong Wang; Heinz C. Schröder; Muhammad Nawaz Tahir; Wolfgang Tremel; Ute Kolb; Werner E.G. Müller

doi:10.1007/s00441-009-0903-x

Silicatein-mediated incorporation of titanium into spicules from the demosponge Suberites domuncula

Filipe Natalio, Enrico Mugnaioli, Matthias Wiens, Xiaohong Wang, Heinz C. Schröder, Muhammad Nawaz Tahir, Wolfgang Tremel, Ute Kolb, Werner E.G. Müller

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO₂) concentrations up to 250 μM. The presence and activity of silicatein in primmorphs has been analyzed by gel electrophoresis and Western blotting. Results of studies by scanning transmission electron microscopy with energy-dispersive X-ray spectroscopy have revealed silica and titania to be co-localized on nanosized spicules. Our findings suggest that the incorporation of titania into the nanosized spicule is enzymatically mediated via active silicatein in an orchestrated mechanism.

Original language	English
Pages (from-to)	429-436
Number of pages	8
Journal	Cell and Tissue Research
Volume	339
Issue number	2
DOIs	https://doi.org/10.1007/s00441-009-0903-x
State	Published - Feb 2010
Externally published	Yes

Bibliographical note

Funding Information:
This research was supported by the Deutsche Forschungsgemeinschaft (Schr 277/10-1), the Bundesministerium für Bildung und Forschung, Germany (Center of Excellence BIOTECmarin), the European Society for Marine Biotechnology, the International Human Frontier Science Program, and the Materials Science Center Mainz (MWFZ). F.Natalio.M.Wiens.H.C.Schröder.W.E.G.Müller(*) Institute for Physiological Chemistry, Department of Applied Molecular Biology, Johannes-Gutenberg-Universität Mainz, Duesbergweg 6, 55099 Mainz, Germany e-mail: [email protected]

Keywords

Nanomaterials
Primmorphs
Silicatein
Sponges
Suberites domuncula (Porifera)

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology
Cell Biology

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title = "Silicatein-mediated incorporation of titanium into spicules from the demosponge Suberites domuncula",

abstract = "Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations up to 250 μM. The presence and activity of silicatein in primmorphs has been analyzed by gel electrophoresis and Western blotting. Results of studies by scanning transmission electron microscopy with energy-dispersive X-ray spectroscopy have revealed silica and titania to be co-localized on nanosized spicules. Our findings suggest that the incorporation of titania into the nanosized spicule is enzymatically mediated via active silicatein in an orchestrated mechanism.",

keywords = "Nanomaterials, Primmorphs, Silicatein, Sponges, Suberites domuncula (Porifera)",

author = "Filipe Natalio and Enrico Mugnaioli and Matthias Wiens and Xiaohong Wang and Schr{\"o}der, {Heinz C.} and Tahir, {Muhammad Nawaz} and Wolfgang Tremel and Ute Kolb and M{\"u}ller, {Werner E.G.}",

note = "Funding Information: This research was supported by the Deutsche Forschungsgemeinschaft (Schr 277/10-1), the Bundesministerium f{\"u}r Bildung und Forschung, Germany (Center of Excellence BIOTECmarin), the European Society for Marine Biotechnology, the International Human Frontier Science Program, and the Materials Science Center Mainz (MWFZ). F.Natalio.M.Wiens.H.C.Schr{\"o}der.W.E.G.M{\"u}ller(*) Institute for Physiological Chemistry, Department of Applied Molecular Biology, Johannes-Gutenberg-Universit{\"a}t Mainz, Duesbergweg 6, 55099 Mainz, Germany e-mail: [email protected]",

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doi = "10.1007/s00441-009-0903-x",

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AU - Natalio, Filipe

AU - Mugnaioli, Enrico

AU - Wiens, Matthias

AU - Wang, Xiaohong

AU - Schröder, Heinz C.

AU - Tahir, Muhammad Nawaz

AU - Tremel, Wolfgang

AU - Kolb, Ute

AU - Müller, Werner E.G.

N1 - Funding Information: This research was supported by the Deutsche Forschungsgemeinschaft (Schr 277/10-1), the Bundesministerium für Bildung und Forschung, Germany (Center of Excellence BIOTECmarin), the European Society for Marine Biotechnology, the International Human Frontier Science Program, and the Materials Science Center Mainz (MWFZ). F.Natalio.M.Wiens.H.C.Schröder.W.E.G.Müller(*) Institute for Physiological Chemistry, Department of Applied Molecular Biology, Johannes-Gutenberg-Universität Mainz, Duesbergweg 6, 55099 Mainz, Germany e-mail: [email protected]

PY - 2010/2

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N2 - Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations up to 250 μM. The presence and activity of silicatein in primmorphs has been analyzed by gel electrophoresis and Western blotting. Results of studies by scanning transmission electron microscopy with energy-dispersive X-ray spectroscopy have revealed silica and titania to be co-localized on nanosized spicules. Our findings suggest that the incorporation of titania into the nanosized spicule is enzymatically mediated via active silicatein in an orchestrated mechanism.

AB - Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations up to 250 μM. The presence and activity of silicatein in primmorphs has been analyzed by gel electrophoresis and Western blotting. Results of studies by scanning transmission electron microscopy with energy-dispersive X-ray spectroscopy have revealed silica and titania to be co-localized on nanosized spicules. Our findings suggest that the incorporation of titania into the nanosized spicule is enzymatically mediated via active silicatein in an orchestrated mechanism.

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Silicatein-mediated incorporation of titanium into spicules from the demosponge Suberites domuncula

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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