Sensitive and rapid electrochemical bioassay of glycosidase activity

Tanin Tangkuaram; Jared Q. Gerlach; Yun Xiang; Abdel Nasser Kawde; Zong Dai; Veer P. Bhavanandan; Jeffrey T. La Belle; Waret Veerasai; Lokesh Joshi; Joseph Wang

doi:10.1039/b605943k

Sensitive and rapid electrochemical bioassay of glycosidase activity

Tanin Tangkuaram, Jared Q. Gerlach, Yun Xiang, Abdel Nasser Kawde, Zong Dai, Veer P. Bhavanandan, Jeffrey T. La Belle, Waret Veerasai, Lokesh Joshi^*, Joseph Wang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

Original language	English
Pages (from-to)	889-891
Number of pages	3
Journal	The Analyst
Volume	131
Issue number	8
DOIs	https://doi.org/10.1039/b605943k
State	Published - 2006
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/b605943k

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title = "Sensitive and rapid electrochemical bioassay of glycosidase activity",

abstract = "Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.",

author = "Tanin Tangkuaram and Gerlach, \{Jared Q.\} and Yun Xiang and Kawde, \{Abdel Nasser\} and Zong Dai and Bhavanandan, \{Veer P.\} and \{La Belle\}, \{Jeffrey T.\} and Waret Veerasai and Lokesh Joshi and Joseph Wang",

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doi = "10.1039/b605943k",

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pages = "889--891",

journal = "The Analyst",

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TY - JOUR

T1 - Sensitive and rapid electrochemical bioassay of glycosidase activity

AU - Tangkuaram, Tanin

AU - Gerlach, Jared Q.

AU - Xiang, Yun

AU - Kawde, Abdel Nasser

AU - Dai, Zong

AU - Bhavanandan, Veer P.

AU - La Belle, Jeffrey T.

AU - Veerasai, Waret

AU - Joshi, Lokesh

AU - Wang, Joseph

PY - 2006

Y1 - 2006

N2 - Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

AB - Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

UR - https://www.scopus.com/pages/publications/33746437088

U2 - 10.1039/b605943k

DO - 10.1039/b605943k

M3 - Article

C2 - 17028720

AN - SCOPUS:33746437088

SN - 0003-2654

VL - 131

SP - 889

EP - 891

JO - The Analyst

JF - The Analyst

IS - 8

ER -

Sensitive and rapid electrochemical bioassay of glycosidase activity

Abstract

ASJC Scopus subject areas

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