Sample loading in gel electrophoresis using adapted 3D printers

Tao Tao; Alexey Kopyl; Yuki Yew; Fawaz El-Dani; Hassan Ali Abid; Thomas Hiscox; Oi Wah Liew; Tuck Wah Ng

doi:10.1016/j.ab.2024.115721

Sample loading in gel electrophoresis using adapted 3D printers

Tao Tao
, Alexey Kopyl
, Yuki Yew
, Fawaz El-Dani
, Hassan Ali Abid
, Thomas Hiscox
, Oi Wah Liew
, Tuck Wah Ng^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.

Original language	English
Article number	115721
Journal	Analytical Biochemistry
Volume	697
DOIs	https://doi.org/10.1016/j.ab.2024.115721
State	Published - Feb 2025
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2024 The Authors

Keywords

3D printer
Gel electrophoresis
Liquid handler
Optical sensor

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2024.115721

Cite this

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title = "Sample loading in gel electrophoresis using adapted 3D printers",

abstract = "In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.",

keywords = "3D printer, Gel electrophoresis, Liquid handler, Optical sensor",

author = "Tao Tao and Alexey Kopyl and Yuki Yew and Fawaz El-Dani and Abid, \{Hassan Ali\} and Thomas Hiscox and Liew, \{Oi Wah\} and Ng, \{Tuck Wah\}",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors",

year = "2025",

month = feb,

doi = "10.1016/j.ab.2024.115721",

language = "English",

volume = "697",

journal = "Analytical Biochemistry",

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T1 - Sample loading in gel electrophoresis using adapted 3D printers

AU - Tao, Tao

AU - Kopyl, Alexey

AU - Yew, Yuki

AU - El-Dani, Fawaz

AU - Abid, Hassan Ali

AU - Hiscox, Thomas

AU - Liew, Oi Wah

AU - Ng, Tuck Wah

PY - 2025/2

Y1 - 2025/2

N2 - In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.

AB - In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.

KW - 3D printer

KW - Gel electrophoresis

KW - Liquid handler

KW - Optical sensor

UR - https://www.scopus.com/pages/publications/85210064193

U2 - 10.1016/j.ab.2024.115721

DO - 10.1016/j.ab.2024.115721

M3 - Article

AN - SCOPUS:85210064193

SN - 0003-2697

VL - 697

JO - Analytical Biochemistry

JF - Analytical Biochemistry

M1 - 115721

ER -

Sample loading in gel electrophoresis using adapted 3D printers

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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Cite this