@inproceedings{bd2e99e2a8e24492b6fb567eb8000874,
title = "Rapid tissue dissociation with microfluidic chip for gene extraction",
abstract = "This paper presents a novel device to disrupt and homogenize tissue samples for the effective extraction of the genomic DNA, total RNA and proteins from tissue samples. This device utilizes a resonant piezo-diaphragm coated with rough sand grains, which effectively homogenizes liver sample (10-50 mg) within 15 s. High yield of genomic DNA (0.98 μg/mg) and intact toal RNA (0.15 μg/mg) are successfully extracted from the dissociated rat liver sample solution. The simplicity of this planar chip would enable potential integration with on-chip RT-PCR for cancer typing and sub-typing using biopsy tumor tissue sample.",
keywords = "DNA/RNA extraction, Sample preparation, Tissue dissociation",
author = "Xu, \{Guo Lin\} and Lee, \{Daniel Yoke San\} and Li, \{Mo Huang\} and Ying, \{Jackie Y.\}",
year = "2009",
language = "English",
isbn = "9780979806421",
series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "1055--1057",
booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",
}
