Rapid tissue dissociation with microfluidic chip for gene extraction

Guo Lin Xu; Daniel Yoke San Lee; Mo Huang Li; Jackie Y. Ying

Rapid tissue dissociation with microfluidic chip for gene extraction

Guo Lin Xu
, Daniel Yoke San Lee
, Mo Huang Li
, Jackie Y. Ying

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

This paper presents a novel device to disrupt and homogenize tissue samples for the effective extraction of the genomic DNA, total RNA and proteins from tissue samples. This device utilizes a resonant piezo-diaphragm coated with rough sand grains, which effectively homogenizes liver sample (10-50 mg) within 15 s. High yield of genomic DNA (0.98 μg/mg) and intact toal RNA (0.15 μg/mg) are successfully extracted from the dissociated rat liver sample solution. The simplicity of this planar chip would enable potential integration with on-chip RT-PCR for cancer typing and sub-typing using biopsy tumor tissue sample.

Original language	English
Title of host publication	Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1055-1057
Number of pages	3
ISBN (Print)	9780979806421
State	Published - 2009
Externally published	Yes
Event	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of Duration: 1 Nov 2009 → 5 Nov 2009

Publication series

Name	Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/Territory	Korea, Republic of
City	Jeju
Period	1/11/09 → 5/11/09

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

DNA/RNA extraction
Sample preparation
Tissue dissociation

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Xu, G. L., Lee, D. Y. S., Li, M. H., & Ying, J. Y. (2009). Rapid tissue dissociation with microfluidic chip for gene extraction. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1055-1057). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Xu, Guo Lin ; Lee, Daniel Yoke San ; Li, Mo Huang et al. / Rapid tissue dissociation with microfluidic chip for gene extraction. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 1055-1057 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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title = "Rapid tissue dissociation with microfluidic chip for gene extraction",

abstract = "This paper presents a novel device to disrupt and homogenize tissue samples for the effective extraction of the genomic DNA, total RNA and proteins from tissue samples. This device utilizes a resonant piezo-diaphragm coated with rough sand grains, which effectively homogenizes liver sample (10-50 mg) within 15 s. High yield of genomic DNA (0.98 μg/mg) and intact toal RNA (0.15 μg/mg) are successfully extracted from the dissociated rat liver sample solution. The simplicity of this planar chip would enable potential integration with on-chip RT-PCR for cancer typing and sub-typing using biopsy tumor tissue sample.",

keywords = "DNA/RNA extraction, Sample preparation, Tissue dissociation",

author = "Xu, \{Guo Lin\} and Lee, \{Daniel Yoke San\} and Li, \{Mo Huang\} and Ying, \{Jackie Y.\}",

year = "2009",

language = "English",

isbn = "9780979806421",

series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "1055--1057",

booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",

}

Xu, GL, Lee, DYS, Li, MH & Ying, JY 2009, Rapid tissue dissociation with microfluidic chip for gene extraction. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 1055-1057, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 1/11/09.

Rapid tissue dissociation with microfluidic chip for gene extraction. / Xu, Guo Lin; Lee, Daniel Yoke San; Li, Mo Huang et al.
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 1055-1057 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Rapid tissue dissociation with microfluidic chip for gene extraction

AU - Xu, Guo Lin

AU - Lee, Daniel Yoke San

AU - Li, Mo Huang

AU - Ying, Jackie Y.

PY - 2009

Y1 - 2009

N2 - This paper presents a novel device to disrupt and homogenize tissue samples for the effective extraction of the genomic DNA, total RNA and proteins from tissue samples. This device utilizes a resonant piezo-diaphragm coated with rough sand grains, which effectively homogenizes liver sample (10-50 mg) within 15 s. High yield of genomic DNA (0.98 μg/mg) and intact toal RNA (0.15 μg/mg) are successfully extracted from the dissociated rat liver sample solution. The simplicity of this planar chip would enable potential integration with on-chip RT-PCR for cancer typing and sub-typing using biopsy tumor tissue sample.

AB - This paper presents a novel device to disrupt and homogenize tissue samples for the effective extraction of the genomic DNA, total RNA and proteins from tissue samples. This device utilizes a resonant piezo-diaphragm coated with rough sand grains, which effectively homogenizes liver sample (10-50 mg) within 15 s. High yield of genomic DNA (0.98 μg/mg) and intact toal RNA (0.15 μg/mg) are successfully extracted from the dissociated rat liver sample solution. The simplicity of this planar chip would enable potential integration with on-chip RT-PCR for cancer typing and sub-typing using biopsy tumor tissue sample.

KW - DNA/RNA extraction

KW - Sample preparation

KW - Tissue dissociation

UR - https://www.scopus.com/pages/publications/84901789474

M3 - Conference contribution

AN - SCOPUS:84901789474

SN - 9780979806421

T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 1055

EP - 1057

BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009

Y2 - 1 November 2009 through 5 November 2009

ER -

Xu GL, Lee DYS, Li MH, Ying JY. Rapid tissue dissociation with microfluidic chip for gene extraction. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 1055-1057. (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Rapid tissue dissociation with microfluidic chip for gene extraction

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

Fingerprint

Cite this