Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method

Sanjiv K. Mishra; William I. Suh; Wasif Farooq; Myounghoon Moon; Anupama Shrivastav; Min S. Park; Ji Won Yang

doi:10.1016/j.biortech.2013.12.077

Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method

Sanjiv K. Mishra
, William I. Suh
, Wasif Farooq
, Myounghoon Moon
, Anupama Shrivastav
, Min S. Park^*
, Ji Won Yang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

390 Scopus citations

Abstract

Identification of novel microalgal strains with high lipid productivity is one of the most important research topics in renewable biofuel research. However, the major bottleneck in the strain screening process is that currently known methods for the estimation of microalgal lipid are laborious and time-consuming. The present study successfully employed sulpho-phospho-vanillin (SPV) colorimetric method for direct quantitative measurement of lipids within liquid microalgal culture. The SPV reacts with lipids to produce a distinct pink color, and its intensity can be quantified using spectrophotometric methods by measuring absorbance at 530nm. This method was employed for a rapid quantification of intracellular lipid contents within Chlorella sp., Monoraphidium sp., Ettlia sp. and Nannochloropsis sp., all of which were found to have lipid contents ranging in between 10% and 30%. Subsequent analysis of the biomass using gas chromatography confirmed that our protocol is highly accurate (R²=0.99).

Original language	English
Pages (from-to)	330-333
Number of pages	4
Journal	Bioresource Technology
Volume	155
DOIs	https://doi.org/10.1016/j.biortech.2013.12.077
State	Published - Mar 2014
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Science, ICT and Future Planning (ABC-2010-0029728).

Keywords

Colorimetric
Gas chromatography
Lipid
Microalgae
Vanillin

ASJC Scopus subject areas

Environmental Engineering
Bioengineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2013.12.077

Cite this

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title = "Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method",

abstract = "Identification of novel microalgal strains with high lipid productivity is one of the most important research topics in renewable biofuel research. However, the major bottleneck in the strain screening process is that currently known methods for the estimation of microalgal lipid are laborious and time-consuming. The present study successfully employed sulpho-phospho-vanillin (SPV) colorimetric method for direct quantitative measurement of lipids within liquid microalgal culture. The SPV reacts with lipids to produce a distinct pink color, and its intensity can be quantified using spectrophotometric methods by measuring absorbance at 530nm. This method was employed for a rapid quantification of intracellular lipid contents within Chlorella sp., Monoraphidium sp., Ettlia sp. and Nannochloropsis sp., all of which were found to have lipid contents ranging in between 10\% and 30\%. Subsequent analysis of the biomass using gas chromatography confirmed that our protocol is highly accurate (R2=0.99).",

keywords = "Colorimetric, Gas chromatography, Lipid, Microalgae, Vanillin",

author = "Mishra, \{Sanjiv K.\} and Suh, \{William I.\} and Wasif Farooq and Myounghoon Moon and Anupama Shrivastav and Park, \{Min S.\} and Yang, \{Ji Won\}",

note = "Funding Information: This work was supported by the Advanced Biomass R\&D Center (ABC) of Korea Grant funded by the Ministry of Science, ICT and Future Planning (ABC-2010-0029728). ",

year = "2014",

month = mar,

doi = "10.1016/j.biortech.2013.12.077",

language = "English",

volume = "155",

pages = "330--333",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd.",

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T1 - Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method

AU - Mishra, Sanjiv K.

AU - Suh, William I.

AU - Farooq, Wasif

AU - Moon, Myounghoon

AU - Shrivastav, Anupama

AU - Park, Min S.

AU - Yang, Ji Won

N1 - Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Science, ICT and Future Planning (ABC-2010-0029728).

PY - 2014/3

Y1 - 2014/3

N2 - Identification of novel microalgal strains with high lipid productivity is one of the most important research topics in renewable biofuel research. However, the major bottleneck in the strain screening process is that currently known methods for the estimation of microalgal lipid are laborious and time-consuming. The present study successfully employed sulpho-phospho-vanillin (SPV) colorimetric method for direct quantitative measurement of lipids within liquid microalgal culture. The SPV reacts with lipids to produce a distinct pink color, and its intensity can be quantified using spectrophotometric methods by measuring absorbance at 530nm. This method was employed for a rapid quantification of intracellular lipid contents within Chlorella sp., Monoraphidium sp., Ettlia sp. and Nannochloropsis sp., all of which were found to have lipid contents ranging in between 10% and 30%. Subsequent analysis of the biomass using gas chromatography confirmed that our protocol is highly accurate (R2=0.99).

AB - Identification of novel microalgal strains with high lipid productivity is one of the most important research topics in renewable biofuel research. However, the major bottleneck in the strain screening process is that currently known methods for the estimation of microalgal lipid are laborious and time-consuming. The present study successfully employed sulpho-phospho-vanillin (SPV) colorimetric method for direct quantitative measurement of lipids within liquid microalgal culture. The SPV reacts with lipids to produce a distinct pink color, and its intensity can be quantified using spectrophotometric methods by measuring absorbance at 530nm. This method was employed for a rapid quantification of intracellular lipid contents within Chlorella sp., Monoraphidium sp., Ettlia sp. and Nannochloropsis sp., all of which were found to have lipid contents ranging in between 10% and 30%. Subsequent analysis of the biomass using gas chromatography confirmed that our protocol is highly accurate (R2=0.99).

KW - Colorimetric

KW - Gas chromatography

KW - Lipid

KW - Microalgae

KW - Vanillin

UR - https://www.scopus.com/pages/publications/84892895468

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DO - 10.1016/j.biortech.2013.12.077

M3 - Article

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AN - SCOPUS:84892895468

SN - 0960-8524

VL - 155

SP - 330

EP - 333

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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Cite this