Quantum-dot/aptamer-based ultrasensitive multi-analyte electrochemical biosensor

Jacob A. Hansen; Joseph Wang; Abdel Nasser Kawde; Yun Xiang; Kurt V. Gothelf; Greg Collins

doi:10.1021/ja060005h

Quantum-dot/aptamer-based ultrasensitive multi-analyte electrochemical biosensor

Jacob A. Hansen
, Joseph Wang^*
, Abdel Nasser Kawde
, Yun Xiang
, Kurt V. Gothelf
, Greg Collins

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

535 Scopus citations

Abstract

The coupling of aptamers with the coding and amplification features of inorganic nanocrystals is shown for the first time to offer a highly sensitive and selective simultaneous bioelectronic detection of several protein targets. This is accomplished in a single-step displacement assay in connection to a self-assembled monolayer of several thiolated aptamers conjugated to proteins carrying different inorganic nanocrystals. Electrochemical stripping detection of the nondisplaced nanocrystal tracers results in a remarkably low (attomole) detection limit, that is, significantly lower than those of existing aptamer biosensors. The new device offers great promise for measuring a large panel of disease markers present at ultralow levels during early stages of the disease progress.

Original language	English
Pages (from-to)	2228-2229
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	128
Issue number	7
DOIs	https://doi.org/10.1021/ja060005h
State	Published - 22 Feb 2006
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja060005h

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title = "Quantum-dot/aptamer-based ultrasensitive multi-analyte electrochemical biosensor",

abstract = "The coupling of aptamers with the coding and amplification features of inorganic nanocrystals is shown for the first time to offer a highly sensitive and selective simultaneous bioelectronic detection of several protein targets. This is accomplished in a single-step displacement assay in connection to a self-assembled monolayer of several thiolated aptamers conjugated to proteins carrying different inorganic nanocrystals. Electrochemical stripping detection of the nondisplaced nanocrystal tracers results in a remarkably low (attomole) detection limit, that is, significantly lower than those of existing aptamer biosensors. The new device offers great promise for measuring a large panel of disease markers present at ultralow levels during early stages of the disease progress.",

author = "Hansen, \{Jacob A.\} and Joseph Wang and Kawde, \{Abdel Nasser\} and Yun Xiang and Gothelf, \{Kurt V.\} and Greg Collins",

year = "2006",

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doi = "10.1021/ja060005h",

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journal = "Journal of the American Chemical Society",

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AU - Hansen, Jacob A.

AU - Wang, Joseph

AU - Kawde, Abdel Nasser

AU - Xiang, Yun

AU - Gothelf, Kurt V.

AU - Collins, Greg

PY - 2006/2/22

Y1 - 2006/2/22

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AB - The coupling of aptamers with the coding and amplification features of inorganic nanocrystals is shown for the first time to offer a highly sensitive and selective simultaneous bioelectronic detection of several protein targets. This is accomplished in a single-step displacement assay in connection to a self-assembled monolayer of several thiolated aptamers conjugated to proteins carrying different inorganic nanocrystals. Electrochemical stripping detection of the nondisplaced nanocrystal tracers results in a remarkably low (attomole) detection limit, that is, significantly lower than those of existing aptamer biosensors. The new device offers great promise for measuring a large panel of disease markers present at ultralow levels during early stages of the disease progress.

UR - https://www.scopus.com/pages/publications/33644534793

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 7

ER -

Quantum-dot/aptamer-based ultrasensitive multi-analyte electrochemical biosensor

Abstract

ASJC Scopus subject areas

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