Abstract
In this study, prolactin gene polymorphism was investigated in Nili- Ravi buffaloes, Sahiwal and Achai cattle breeds, 100 per group, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Only genotype GG was observed in the case of Nili-Ravi buffaloes. In Sahiwal and Achai cattle, three genotypes were found, AA, AG and GG: the frequency of these genotypes were 72%, 18% and 10% in Sahiwal cattle and 44%, 34% and 22% in Achai cattle, respectively. The frequency of genotype AA was found to be higher in both cattle breeds. Results of chi-square test at P<0.05 revealed that animals of Achai cattle were in Hardy-Weinberg equilibrium, whereas Sahiwal cattle were found to be deviating.
| Original language | English |
|---|---|
| Pages (from-to) | 20-24 |
| Number of pages | 5 |
| Journal | International Journal of Dairy Technology |
| Volume | 66 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2013 |
| Externally published | Yes |
Keywords
- Achai
- Nili-Ravi
- PCR-RFLP
- Polymorphism
- Sahiwal
ASJC Scopus subject areas
- Food Science
- Bioengineering
- Process Chemistry and Technology
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