Porcine cholecyst derived extracellular matrix (p-CEM) for reconstruction of full thickness skin wounds in rats

An acellular cholecyst derived extracellular matrix (p-CEM) of porcine origin was prepared using anionic biological detergent. Healing potential of (p-CEM) was compared with commercially available collagen sheet (b-CS) and open wound (C) in full-thickness skin wounds in rats. Thirty six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full-thickness skin wound (20×20mm²) was produced on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (p-CEM). Healing was evaluated on the basis of wound contraction, gross, hematological, immunological and histopathological observations. On critical analysis of the observed parameters it was found that p-CEM has healing potential and shown better healing response than commercially available collagen sheet to reconstruct full thickness skin wounds in rats.