Polyester sulphonic acid interstitial nanocomposite platform for peroxide biosensor

Amir Al-Ahmed; Peter M. Ndangili; Nazeem Jahed; Priscilla G.L. Baker; Emmanuel I. Iwuoha

doi:10.3390/s91209965

Polyester sulphonic acid interstitial nanocomposite platform for peroxide biosensor

Amir Al-Ahmed
, Peter M. Ndangili
, Nazeem Jahed
, Priscilla G.L. Baker
, Emmanuel I. Iwuoha

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A novel enzyme immobilization platform was prepared on a platinum disk working electrode by polymerizing aniline inside the interstitial pores of polyester sulphonic acid sodium salt (PESA). Scanning electron microscopy study showed the formation of homogeneous sulphonated polyaniline (PANI) nanotubes (~90 nm) and thermogravimetric analysis (TGA) confirmed that the nanotubes were stable up to 230 °C. The PANI:PESA nanocomposite showed a quasi-reversible redox behaviour in phosphate buffer saline. Horseradish peroxidase (HRP) was immobilized on to this modified electrode for hydrogen peroxide detection. The biosensor gave a sensitivity of 1.33 μA (μM)^-1 and a detection limit of 0.185 μM for H₂O₂. Stability experiments showed that the biosensor retained more than 64% of its initial sensitivity over four days of storage at 4 °C.

Original language	English
Pages (from-to)	9965-9976
Number of pages	12
Journal	Sensors
Volume	9
Issue number	12
DOIs	https://doi.org/10.3390/s91209965
State	Published - Dec 2009
Externally published	Yes

Keywords

Electrochemical polymerisation
HRP
Immobilization of enzyme
Peroxide biosensors
Polyaniline nanotubes
Polyester sulphonic acid

ASJC Scopus subject areas

Analytical Chemistry
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering

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10.3390/s91209965

Cite this

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title = "Polyester sulphonic acid interstitial nanocomposite platform for peroxide biosensor",

abstract = "A novel enzyme immobilization platform was prepared on a platinum disk working electrode by polymerizing aniline inside the interstitial pores of polyester sulphonic acid sodium salt (PESA). Scanning electron microscopy study showed the formation of homogeneous sulphonated polyaniline (PANI) nanotubes (\textasciitilde{}90 nm) and thermogravimetric analysis (TGA) confirmed that the nanotubes were stable up to 230 °C. The PANI:PESA nanocomposite showed a quasi-reversible redox behaviour in phosphate buffer saline. Horseradish peroxidase (HRP) was immobilized on to this modified electrode for hydrogen peroxide detection. The biosensor gave a sensitivity of 1.33 μA (μM)-1 and a detection limit of 0.185 μM for H2O2. Stability experiments showed that the biosensor retained more than 64\% of its initial sensitivity over four days of storage at 4 °C.",

keywords = "Electrochemical polymerisation, HRP, Immobilization of enzyme, Peroxide biosensors, Polyaniline nanotubes, Polyester sulphonic acid",

author = "Amir Al-Ahmed and Ndangili, \{Peter M.\} and Nazeem Jahed and Baker, \{Priscilla G.L.\} and Iwuoha, \{Emmanuel I.\}",

year = "2009",

month = dec,

doi = "10.3390/s91209965",

language = "English",

volume = "9",

pages = "9965--9976",

journal = "Sensors",

issn = "1424-8220",

number = "12",

}

TY - JOUR

T1 - Polyester sulphonic acid interstitial nanocomposite platform for peroxide biosensor

AU - Al-Ahmed, Amir

AU - Ndangili, Peter M.

AU - Jahed, Nazeem

AU - Baker, Priscilla G.L.

AU - Iwuoha, Emmanuel I.

PY - 2009/12

Y1 - 2009/12

N2 - A novel enzyme immobilization platform was prepared on a platinum disk working electrode by polymerizing aniline inside the interstitial pores of polyester sulphonic acid sodium salt (PESA). Scanning electron microscopy study showed the formation of homogeneous sulphonated polyaniline (PANI) nanotubes (~90 nm) and thermogravimetric analysis (TGA) confirmed that the nanotubes were stable up to 230 °C. The PANI:PESA nanocomposite showed a quasi-reversible redox behaviour in phosphate buffer saline. Horseradish peroxidase (HRP) was immobilized on to this modified electrode for hydrogen peroxide detection. The biosensor gave a sensitivity of 1.33 μA (μM)-1 and a detection limit of 0.185 μM for H2O2. Stability experiments showed that the biosensor retained more than 64% of its initial sensitivity over four days of storage at 4 °C.

AB - A novel enzyme immobilization platform was prepared on a platinum disk working electrode by polymerizing aniline inside the interstitial pores of polyester sulphonic acid sodium salt (PESA). Scanning electron microscopy study showed the formation of homogeneous sulphonated polyaniline (PANI) nanotubes (~90 nm) and thermogravimetric analysis (TGA) confirmed that the nanotubes were stable up to 230 °C. The PANI:PESA nanocomposite showed a quasi-reversible redox behaviour in phosphate buffer saline. Horseradish peroxidase (HRP) was immobilized on to this modified electrode for hydrogen peroxide detection. The biosensor gave a sensitivity of 1.33 μA (μM)-1 and a detection limit of 0.185 μM for H2O2. Stability experiments showed that the biosensor retained more than 64% of its initial sensitivity over four days of storage at 4 °C.

KW - Electrochemical polymerisation

KW - HRP

KW - Immobilization of enzyme

KW - Peroxide biosensors

KW - Polyaniline nanotubes

KW - Polyester sulphonic acid

UR - https://www.scopus.com/pages/publications/77950242327

U2 - 10.3390/s91209965

DO - 10.3390/s91209965

M3 - Article

AN - SCOPUS:77950242327

SN - 1424-8220

VL - 9

SP - 9965

EP - 9976

JO - Sensors

JF - Sensors

IS - 12

ER -

Polyester sulphonic acid interstitial nanocomposite platform for peroxide biosensor

Abstract

Keywords

ASJC Scopus subject areas

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