Platform for highly sensitive alkaline phosphatase-based immunosensors using 1-naphthyl phosphate and an avidin-modified indium tin oxide electrode

Md Abdul Aziz; Kyungmin Jo; Md Abdul Qaium; Chan Hwa Huh; In Seok Hong; Haesik Yang

doi:10.1002/elan.200904641

Platform for highly sensitive alkaline phosphatase-based immunosensors using 1-naphthyl phosphate and an avidin-modified indium tin oxide electrode

Md Abdul Aziz
, Kyungmin Jo
, Md Abdul Qaium
, Chan Hwa Huh
, In Seok Hong
, Haesik Yang

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We report a versatile platform for highly sensitive alkaline phosphatase (ALP)-based electrochemical biosensors that uses an avidin-modified indium tin oxide (ITO) electrode as a sensing electrode and 1-naphthyl phosphate (NPP) as an ALP substrate. Almost no electrocatalytic activity of NPP and good electrocatalytic activity of 1-naphthol (ALP product) on the ITO electrodes allow a high signal-to-background ratio. The effective surface covering of avidin on the ITO electrodes allows very low levels of nonspecific binding of proteins to the sensing electrodes. The platform technology is used to detect mouse IgG with a detection limit of 1.0 pg/mL.

Original language	English
Pages (from-to)	2160-2164
Number of pages	5
Journal	Electroanalysis
Volume	21
Issue number	19
DOIs	https://doi.org/10.1002/elan.200904641
State	Published - Oct 2009
Externally published	Yes

Keywords

Alkaline phosphatase
Avidin
Biosensors
Enzymes
Immunosensor
Nonspecific binding

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

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10.1002/elan.200904641

Cite this

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title = "Platform for highly sensitive alkaline phosphatase-based immunosensors using 1-naphthyl phosphate and an avidin-modified indium tin oxide electrode",

abstract = "We report a versatile platform for highly sensitive alkaline phosphatase (ALP)-based electrochemical biosensors that uses an avidin-modified indium tin oxide (ITO) electrode as a sensing electrode and 1-naphthyl phosphate (NPP) as an ALP substrate. Almost no electrocatalytic activity of NPP and good electrocatalytic activity of 1-naphthol (ALP product) on the ITO electrodes allow a high signal-to-background ratio. The effective surface covering of avidin on the ITO electrodes allows very low levels of nonspecific binding of proteins to the sensing electrodes. The platform technology is used to detect mouse IgG with a detection limit of 1.0 pg/mL.",

keywords = "Alkaline phosphatase, Avidin, Biosensors, Enzymes, Immunosensor, Nonspecific binding",

author = "Aziz, \{Md Abdul\} and Kyungmin Jo and Qaium, \{Md Abdul\} and Huh, \{Chan Hwa\} and Hong, \{In Seok\} and Haesik Yang",

year = "2009",

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doi = "10.1002/elan.200904641",

language = "English",

volume = "21",

pages = "2160--2164",

journal = "Electroanalysis",

issn = "1040-0397",

publisher = "Wiley-VCH Verlag",

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TY - JOUR

T1 - Platform for highly sensitive alkaline phosphatase-based immunosensors using 1-naphthyl phosphate and an avidin-modified indium tin oxide electrode

AU - Aziz, Md Abdul

AU - Jo, Kyungmin

AU - Qaium, Md Abdul

AU - Huh, Chan Hwa

AU - Hong, In Seok

AU - Yang, Haesik

PY - 2009/10

Y1 - 2009/10

N2 - We report a versatile platform for highly sensitive alkaline phosphatase (ALP)-based electrochemical biosensors that uses an avidin-modified indium tin oxide (ITO) electrode as a sensing electrode and 1-naphthyl phosphate (NPP) as an ALP substrate. Almost no electrocatalytic activity of NPP and good electrocatalytic activity of 1-naphthol (ALP product) on the ITO electrodes allow a high signal-to-background ratio. The effective surface covering of avidin on the ITO electrodes allows very low levels of nonspecific binding of proteins to the sensing electrodes. The platform technology is used to detect mouse IgG with a detection limit of 1.0 pg/mL.

AB - We report a versatile platform for highly sensitive alkaline phosphatase (ALP)-based electrochemical biosensors that uses an avidin-modified indium tin oxide (ITO) electrode as a sensing electrode and 1-naphthyl phosphate (NPP) as an ALP substrate. Almost no electrocatalytic activity of NPP and good electrocatalytic activity of 1-naphthol (ALP product) on the ITO electrodes allow a high signal-to-background ratio. The effective surface covering of avidin on the ITO electrodes allows very low levels of nonspecific binding of proteins to the sensing electrodes. The platform technology is used to detect mouse IgG with a detection limit of 1.0 pg/mL.

KW - Alkaline phosphatase

KW - Avidin

KW - Biosensors

KW - Enzymes

KW - Immunosensor

KW - Nonspecific binding

UR - https://www.scopus.com/pages/publications/70349847689

U2 - 10.1002/elan.200904641

DO - 10.1002/elan.200904641

M3 - Article

AN - SCOPUS:70349847689

SN - 1040-0397

VL - 21

SP - 2160

EP - 2164

JO - Electroanalysis

JF - Electroanalysis

IS - 19

ER -

Platform for highly sensitive alkaline phosphatase-based immunosensors using 1-naphthyl phosphate and an avidin-modified indium tin oxide electrode

Abstract

Keywords

ASJC Scopus subject areas

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