Physico-chemical and microbial characterization of compartment-wise profiles in an anammox baffled reactor

In this study, compartment-wise investigation of an anammox baffled reactor (AnBR) was performed. The AnBR achieved steady-state conditions after a start-up period of ∼50 days and achieved NH₄ and NO₂ conversion percentages of 88.5 and 99.3%, respectively. Examination of the nitrogen mass balance revealed that an AnBR with a two-compartment configuration was sufficient for nitrogen loading rates (NLRs) ranging from 0.125 to 1.975 kg N/m³/d and resulted in a nitrogen removal efficiency (NRE) of 86.7–93.7%. Higher NLRs (4.04–5.05 kg N/m³/d) required four compartments to achieve an NRE of 82.2–87.1%. Further, an overall NLR increase of up to 5.93 ± 0.23 kg N/m³/d resulted in complete AnBR failure. The maximum nitrogen removal rate was consistently recorded in the 1^st compartment for all NLRs examined; as a result, this compartment exhibited the highest bacterial activity. Biomass concentration, specific anammox activity, extracellular polymeric substances, and average granule diameter in the 1^st compartment with an overall NLR of 0.05 kg N/m³/d were estimated to be 11.2 g_VSS/L, 0.03 mg N/g_VSS/h, 84.3 mg/g_VSS and 0.65 mm, respectively. These values increased to 26.1 g_VSS/L, 11.80 mg N/g_VSS/h, 242.1 mg/g_VSS and 2.31 mm, respectively, when the overall NLR was incremented to 4.04 kg N/m³/d. However, a gradual reduction in bacterial activity was observed from the 1^st to the 5^th compartment. The microbial community analysis indicated that the dominant phyla in the 1^st compartment (NLR of 0.252 kg N/m³/d) with the highest nitrogen removal were Chloroflexi (38.13%), Planctomycetes (22.62%), and Proteobacteria (14.75%).