Optimization of cnts production using full factorial design and its advanced application in protein purification

N. M. Mubarak; Faridah Yusof; M. F. Alkhatib; Emad Ameen; M. Khalid; Al Saadi Mohammed; A. Muataz; I. Y. Qudsieh; W. Rashmi

doi:10.1142/S0219581X10006648

Optimization of cnts production using full factorial design and its advanced application in protein purification

N. M. Mubarak, Faridah Yusof, M. F. Alkhatib, Emad Ameen, M. Khalid, Al Saadi Mohammed, A. Muataz, I. Y. Qudsieh, W. Rashmi

King Fahd University of Petroleum & Minerals

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Carbon nanotubes (CNTs) have been successfully synthesized by using in-house fabricated Double Stage Chemical Vapor Deposition (DS-CVD) technique, using acetylene (C₂H₂) and hydrogen (H₂) as the precursor gases. The purity, morphology and the structure of CNTs were then characterized using Field Emission Scanning Microscope (FESEM), Transmission Electron Microscope (TEM) and Thermogravimetric Analysis (TGA). The effects of the process parameters were examined whereby the experimental design of the investigation was conducted using Design Expert^® Version 6.0.8. The statistical analysis reveals that the optimized conditions for the best CNTs yield production at 850°C reaction temperature, 60 min reaction time, with gas flow rates at 40 and 150 ml/min for C₂H₂ and H ₂, respectively. The CNTs produced were successfully used as column chromatographic media. Due to its nanosized structured dimension, CNTs' have tremendously large surface area and that lead to highly efficient protein purification. Skim latex protein has been used as the model protein and we aim to recover useful proteins and enzymes from this known wasteful material. During the purification, the process parameters such as pH and ionic strength of the running buffer were optimized to enhance protein purification. Results reveal that CNTs behave efficiently as a hydrophobic interaction chromatographic media.

Original language	English
Pages (from-to)	181-192
Number of pages	12
Journal	International Journal of Nanoscience
Volume	9
Issue number	3
DOIs	https://doi.org/10.1142/S0219581X10006648
State	Published - Jun 2010

Keywords

CNTs
DS-CVD
functionalization
protein purification
skim latex serum

ASJC Scopus subject areas

Biotechnology
Bioengineering
General Materials Science
Condensed Matter Physics
Computer Science Applications
Electrical and Electronic Engineering

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10.1142/S0219581X10006648

Cite this

@article{6295fd7848d44b2484f8ecdbda9dcc1c,

title = "Optimization of cnts production using full factorial design and its advanced application in protein purification",

abstract = "Carbon nanotubes (CNTs) have been successfully synthesized by using in-house fabricated Double Stage Chemical Vapor Deposition (DS-CVD) technique, using acetylene (C2H2) and hydrogen (H2) as the precursor gases. The purity, morphology and the structure of CNTs were then characterized using Field Emission Scanning Microscope (FESEM), Transmission Electron Microscope (TEM) and Thermogravimetric Analysis (TGA). The effects of the process parameters were examined whereby the experimental design of the investigation was conducted using Design Expert{\textregistered} Version 6.0.8. The statistical analysis reveals that the optimized conditions for the best CNTs yield production at 850°C reaction temperature, 60 min reaction time, with gas flow rates at 40 and 150 ml/min for C2H2 and H 2, respectively. The CNTs produced were successfully used as column chromatographic media. Due to its nanosized structured dimension, CNTs' have tremendously large surface area and that lead to highly efficient protein purification. Skim latex protein has been used as the model protein and we aim to recover useful proteins and enzymes from this known wasteful material. During the purification, the process parameters such as pH and ionic strength of the running buffer were optimized to enhance protein purification. Results reveal that CNTs behave efficiently as a hydrophobic interaction chromatographic media.",

keywords = "CNTs, DS-CVD, functionalization, protein purification, skim latex serum",

author = "Mubarak, \{N. M.\} and Faridah Yusof and Alkhatib, \{M. F.\} and Emad Ameen and M. Khalid and Mohammed, \{Al Saadi\} and A. Muataz and Qudsieh, \{I. Y.\} and W. Rashmi",

year = "2010",

month = jun,

doi = "10.1142/S0219581X10006648",

language = "English",

volume = "9",

pages = "181--192",

journal = "International Journal of Nanoscience",

issn = "0219-581X",

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TY - JOUR

T1 - Optimization of cnts production using full factorial design and its advanced application in protein purification

AU - Mubarak, N. M.

AU - Yusof, Faridah

AU - Alkhatib, M. F.

AU - Ameen, Emad

AU - Khalid, M.

AU - Mohammed, Al Saadi

AU - Muataz, A.

AU - Qudsieh, I. Y.

AU - Rashmi, W.

PY - 2010/6

Y1 - 2010/6

N2 - Carbon nanotubes (CNTs) have been successfully synthesized by using in-house fabricated Double Stage Chemical Vapor Deposition (DS-CVD) technique, using acetylene (C2H2) and hydrogen (H2) as the precursor gases. The purity, morphology and the structure of CNTs were then characterized using Field Emission Scanning Microscope (FESEM), Transmission Electron Microscope (TEM) and Thermogravimetric Analysis (TGA). The effects of the process parameters were examined whereby the experimental design of the investigation was conducted using Design Expert® Version 6.0.8. The statistical analysis reveals that the optimized conditions for the best CNTs yield production at 850°C reaction temperature, 60 min reaction time, with gas flow rates at 40 and 150 ml/min for C2H2 and H 2, respectively. The CNTs produced were successfully used as column chromatographic media. Due to its nanosized structured dimension, CNTs' have tremendously large surface area and that lead to highly efficient protein purification. Skim latex protein has been used as the model protein and we aim to recover useful proteins and enzymes from this known wasteful material. During the purification, the process parameters such as pH and ionic strength of the running buffer were optimized to enhance protein purification. Results reveal that CNTs behave efficiently as a hydrophobic interaction chromatographic media.

AB - Carbon nanotubes (CNTs) have been successfully synthesized by using in-house fabricated Double Stage Chemical Vapor Deposition (DS-CVD) technique, using acetylene (C2H2) and hydrogen (H2) as the precursor gases. The purity, morphology and the structure of CNTs were then characterized using Field Emission Scanning Microscope (FESEM), Transmission Electron Microscope (TEM) and Thermogravimetric Analysis (TGA). The effects of the process parameters were examined whereby the experimental design of the investigation was conducted using Design Expert® Version 6.0.8. The statistical analysis reveals that the optimized conditions for the best CNTs yield production at 850°C reaction temperature, 60 min reaction time, with gas flow rates at 40 and 150 ml/min for C2H2 and H 2, respectively. The CNTs produced were successfully used as column chromatographic media. Due to its nanosized structured dimension, CNTs' have tremendously large surface area and that lead to highly efficient protein purification. Skim latex protein has been used as the model protein and we aim to recover useful proteins and enzymes from this known wasteful material. During the purification, the process parameters such as pH and ionic strength of the running buffer were optimized to enhance protein purification. Results reveal that CNTs behave efficiently as a hydrophobic interaction chromatographic media.

KW - CNTs

KW - DS-CVD

KW - functionalization

KW - protein purification

KW - skim latex serum

UR - https://www.scopus.com/pages/publications/78649263319

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DO - 10.1142/S0219581X10006648

M3 - Article

AN - SCOPUS:78649263319

SN - 0219-581X

VL - 9

SP - 181

EP - 192

JO - International Journal of Nanoscience

JF - International Journal of Nanoscience

IS - 3

ER -

Optimization of cnts production using full factorial design and its advanced application in protein purification

Abstract

Keywords

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