Novel therapeutic biosensor for indinavir-A protease inhibitor antiretroviral drug

A. Ignaszak; N. Hendricks; T. Waryo; E. Songa; N. Jahed; R. Ngece; A. Al-Ahmed; B. Kgarebe; P. Baker; E. I. Iwuoha

doi:10.1016/j.jpba.2008.10.025

Novel therapeutic biosensor for indinavir-A protease inhibitor antiretroviral drug

A. Ignaszak
, N. Hendricks
, T. Waryo
, E. Songa
, N. Jahed
, R. Ngece
, A. Al-Ahmed
, B. Kgarebe
, P. Baker
, E. I. Iwuoha^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

An amperometric drug metabolism biosensor consisting of cytochrome P450-3A4 (CYP3A4) encapsulated in a didodecyldimethylammonium bromide (DDAB) vesicular system on a Pt disk electrode was developed for the determination of indinavir, a protease inhibitor antiretroviral drug. Cyclic, square wave and pulse voltammetric responses of the bioelectrode showed quasi-reversible electrochemistry of the Fe³⁺/Fe²⁺ redox species of the heme thiolate CYP3A4 enzyme under aerobic and anaerobic conditions. The biosensor exhibited excellent response to indinavir with a detection limit and response time of 6.158 × 10^-2 mg L^-1, and 40 s, respectively. The detection limit is well below the plasma concentration of indinavir (8 h after intake) which range from 0.13 to 8.6 mg L^-1.

Original language	English
Pages (from-to)	498-501
Number of pages	4
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	49
Issue number	2
DOIs	https://doi.org/10.1016/j.jpba.2008.10.025
State	Published - 20 Feb 2009
Externally published	Yes

Bibliographical note

Funding Information:
This study was funded by grants from the National Research Foundation (NRF) of South Africa and the Medical Research Council (MRC) of South Africa.

Keywords

Amperometric biosensor
Bioelectrode
CYP3A4
Drug metabolism biosensor
Indinavir

ASJC Scopus subject areas

Analytical Chemistry
Pharmaceutical Science
Drug Discovery
Spectroscopy
Clinical Biochemistry

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10.1016/j.jpba.2008.10.025

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title = "Novel therapeutic biosensor for indinavir-A protease inhibitor antiretroviral drug",

abstract = "An amperometric drug metabolism biosensor consisting of cytochrome P450-3A4 (CYP3A4) encapsulated in a didodecyldimethylammonium bromide (DDAB) vesicular system on a Pt disk electrode was developed for the determination of indinavir, a protease inhibitor antiretroviral drug. Cyclic, square wave and pulse voltammetric responses of the bioelectrode showed quasi-reversible electrochemistry of the Fe3+/Fe2+ redox species of the heme thiolate CYP3A4 enzyme under aerobic and anaerobic conditions. The biosensor exhibited excellent response to indinavir with a detection limit and response time of 6.158 × 10-2 mg L-1, and 40 s, respectively. The detection limit is well below the plasma concentration of indinavir (8 h after intake) which range from 0.13 to 8.6 mg L-1.",

keywords = "Amperometric biosensor, Bioelectrode, CYP3A4, Drug metabolism biosensor, Indinavir",

author = "A. Ignaszak and N. Hendricks and T. Waryo and E. Songa and N. Jahed and R. Ngece and A. Al-Ahmed and B. Kgarebe and P. Baker and Iwuoha, \{E. I.\}",

note = "Funding Information: This study was funded by grants from the National Research Foundation (NRF) of South Africa and the Medical Research Council (MRC) of South Africa.",

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doi = "10.1016/j.jpba.2008.10.025",

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AU - Ignaszak, A.

AU - Hendricks, N.

AU - Waryo, T.

AU - Songa, E.

AU - Jahed, N.

AU - Ngece, R.

AU - Al-Ahmed, A.

AU - Kgarebe, B.

AU - Baker, P.

AU - Iwuoha, E. I.

N1 - Funding Information: This study was funded by grants from the National Research Foundation (NRF) of South Africa and the Medical Research Council (MRC) of South Africa.

PY - 2009/2/20

Y1 - 2009/2/20

N2 - An amperometric drug metabolism biosensor consisting of cytochrome P450-3A4 (CYP3A4) encapsulated in a didodecyldimethylammonium bromide (DDAB) vesicular system on a Pt disk electrode was developed for the determination of indinavir, a protease inhibitor antiretroviral drug. Cyclic, square wave and pulse voltammetric responses of the bioelectrode showed quasi-reversible electrochemistry of the Fe3+/Fe2+ redox species of the heme thiolate CYP3A4 enzyme under aerobic and anaerobic conditions. The biosensor exhibited excellent response to indinavir with a detection limit and response time of 6.158 × 10-2 mg L-1, and 40 s, respectively. The detection limit is well below the plasma concentration of indinavir (8 h after intake) which range from 0.13 to 8.6 mg L-1.

AB - An amperometric drug metabolism biosensor consisting of cytochrome P450-3A4 (CYP3A4) encapsulated in a didodecyldimethylammonium bromide (DDAB) vesicular system on a Pt disk electrode was developed for the determination of indinavir, a protease inhibitor antiretroviral drug. Cyclic, square wave and pulse voltammetric responses of the bioelectrode showed quasi-reversible electrochemistry of the Fe3+/Fe2+ redox species of the heme thiolate CYP3A4 enzyme under aerobic and anaerobic conditions. The biosensor exhibited excellent response to indinavir with a detection limit and response time of 6.158 × 10-2 mg L-1, and 40 s, respectively. The detection limit is well below the plasma concentration of indinavir (8 h after intake) which range from 0.13 to 8.6 mg L-1.

KW - Amperometric biosensor

KW - Bioelectrode

KW - CYP3A4

KW - Drug metabolism biosensor

KW - Indinavir

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DO - 10.1016/j.jpba.2008.10.025

M3 - Article

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SN - 0731-7085

VL - 49

SP - 498

EP - 501

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

IS - 2

ER -

Novel therapeutic biosensor for indinavir-A protease inhibitor antiretroviral drug

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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