Nanoprobe-based genetic testing

Yanbing Zu; Min Han Tan; Balram Chowbay; Soo Chin Lee; Huiling Yap; Ming Ta Michael Lee; Liang Suei Lu; Chun Ping Chang; Jackie Y. Ying

doi:10.1016/j.nantod.2014.04.003

Nanoprobe-based genetic testing

Yanbing Zu
, Min Han Tan
, Balram Chowbay
, Soo Chin Lee
, Huiling Yap
, Ming Ta Michael Lee
, Liang Suei Lu
, Chun Ping Chang
, Jackie Y. Ying^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Summary Plasmonic nanoprobes functionalized with morpholino oligos have been developed for genotyping of single-nucleotide polymorphisms (SNPs) based on colorimetric signals that could be easily visualized. The assay was conducted with a standard thermal cycler that would be available in any molecular diagnostic laboratory, facilitating assay deployment and allowing for cost-effective genetic testing with rapid turnaround time. Specifically, a warfarin sensitivity testing kit gauging three SNPs has been created and validated with 249 human genomic DNA samples in four independent institutes, demonstrating the robustness of our new platform.

Original language	English
Pages (from-to)	166-171
Number of pages	6
Journal	Nano Today
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/j.nantod.2014.04.003
State	Published - Apr 2014
Externally published	Yes

Keywords

Genotyping
Morpholino
Nanoparticle
Pharmacogenomics
Warfarin

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
General Materials Science
Pharmaceutical Science

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10.1016/j.nantod.2014.04.003

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title = "Nanoprobe-based genetic testing",

abstract = "Summary Plasmonic nanoprobes functionalized with morpholino oligos have been developed for genotyping of single-nucleotide polymorphisms (SNPs) based on colorimetric signals that could be easily visualized. The assay was conducted with a standard thermal cycler that would be available in any molecular diagnostic laboratory, facilitating assay deployment and allowing for cost-effective genetic testing with rapid turnaround time. Specifically, a warfarin sensitivity testing kit gauging three SNPs has been created and validated with 249 human genomic DNA samples in four independent institutes, demonstrating the robustness of our new platform.",

keywords = "Genotyping, Morpholino, Nanoparticle, Pharmacogenomics, Warfarin",

author = "Yanbing Zu and Tan, \{Min Han\} and Balram Chowbay and Lee, \{Soo Chin\} and Huiling Yap and Lee, \{Ming Ta Michael\} and Lu, \{Liang Suei\} and Chang, \{Chun Ping\} and Ying, \{Jackie Y.\}",

year = "2014",

month = apr,

doi = "10.1016/j.nantod.2014.04.003",

language = "English",

volume = "9",

pages = "166--171",

journal = "Nano Today",

issn = "1748-0132",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Nanoprobe-based genetic testing

AU - Zu, Yanbing

AU - Tan, Min Han

AU - Chowbay, Balram

AU - Lee, Soo Chin

AU - Yap, Huiling

AU - Lee, Ming Ta Michael

AU - Lu, Liang Suei

AU - Chang, Chun Ping

AU - Ying, Jackie Y.

PY - 2014/4

Y1 - 2014/4

N2 - Summary Plasmonic nanoprobes functionalized with morpholino oligos have been developed for genotyping of single-nucleotide polymorphisms (SNPs) based on colorimetric signals that could be easily visualized. The assay was conducted with a standard thermal cycler that would be available in any molecular diagnostic laboratory, facilitating assay deployment and allowing for cost-effective genetic testing with rapid turnaround time. Specifically, a warfarin sensitivity testing kit gauging three SNPs has been created and validated with 249 human genomic DNA samples in four independent institutes, demonstrating the robustness of our new platform.

AB - Summary Plasmonic nanoprobes functionalized with morpholino oligos have been developed for genotyping of single-nucleotide polymorphisms (SNPs) based on colorimetric signals that could be easily visualized. The assay was conducted with a standard thermal cycler that would be available in any molecular diagnostic laboratory, facilitating assay deployment and allowing for cost-effective genetic testing with rapid turnaround time. Specifically, a warfarin sensitivity testing kit gauging three SNPs has been created and validated with 249 human genomic DNA samples in four independent institutes, demonstrating the robustness of our new platform.

KW - Genotyping

KW - Morpholino

KW - Nanoparticle

KW - Pharmacogenomics

KW - Warfarin

UR - https://www.scopus.com/pages/publications/84902539595

U2 - 10.1016/j.nantod.2014.04.003

DO - 10.1016/j.nantod.2014.04.003

M3 - Article

AN - SCOPUS:84902539595

SN - 1748-0132

VL - 9

SP - 166

EP - 171

JO - Nano Today

JF - Nano Today

IS - 2

ER -

Nanoprobe-based genetic testing

Abstract

Keywords

ASJC Scopus subject areas

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