Nanoparticle-based sensing of glycan-lectin interactions

Zong Dai; Abdel Nasser Kawde; Yun Xiang; Jeffrey T. La Belle; Jared Gerlach; Veer P. Bhavanandan; Lokesh Joshi; Joseph Wang

doi:10.1021/ja063565p

Nanoparticle-based sensing of glycan-lectin interactions

Zong Dai
, Abdel Nasser Kawde
, Yun Xiang
, Jeffrey T. La Belle
, Jared Gerlach
, Veer P. Bhavanandan
, Lokesh Joshi^*
, Joseph Wang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

Here we present the first report on nanoparticle-based biosensing of glycan markers of diseases. The protocol relies on the competition between a nanocrystal (CdS)-tagged sugar and the target sugar for the binding sites of surface-confined lectin and monitoring the extent of competition through highly sensitive electrochemical detection of the captured nanocrystal. This development is expected to allow decentralized detection of carbohydrate moieties and lectin-carbohydrate interactions to be performed more rapidly, sensitively, inexpensively, and reliably.

Original language	English
Pages (from-to)	10018-10019
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	128
Issue number	31
DOIs	https://doi.org/10.1021/ja063565p
State	Published - 9 Aug 2006
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja063565p

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title = "Nanoparticle-based sensing of glycan-lectin interactions",

abstract = "Here we present the first report on nanoparticle-based biosensing of glycan markers of diseases. The protocol relies on the competition between a nanocrystal (CdS)-tagged sugar and the target sugar for the binding sites of surface-confined lectin and monitoring the extent of competition through highly sensitive electrochemical detection of the captured nanocrystal. This development is expected to allow decentralized detection of carbohydrate moieties and lectin-carbohydrate interactions to be performed more rapidly, sensitively, inexpensively, and reliably.",

author = "Zong Dai and Kawde, \{Abdel Nasser\} and Yun Xiang and \{La Belle\}, \{Jeffrey T.\} and Jared Gerlach and Bhavanandan, \{Veer P.\} and Lokesh Joshi and Joseph Wang",

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AU - Dai, Zong

AU - Kawde, Abdel Nasser

AU - Xiang, Yun

AU - La Belle, Jeffrey T.

AU - Gerlach, Jared

AU - Bhavanandan, Veer P.

AU - Joshi, Lokesh

AU - Wang, Joseph

PY - 2006/8/9

Y1 - 2006/8/9

N2 - Here we present the first report on nanoparticle-based biosensing of glycan markers of diseases. The protocol relies on the competition between a nanocrystal (CdS)-tagged sugar and the target sugar for the binding sites of surface-confined lectin and monitoring the extent of competition through highly sensitive electrochemical detection of the captured nanocrystal. This development is expected to allow decentralized detection of carbohydrate moieties and lectin-carbohydrate interactions to be performed more rapidly, sensitively, inexpensively, and reliably.

AB - Here we present the first report on nanoparticle-based biosensing of glycan markers of diseases. The protocol relies on the competition between a nanocrystal (CdS)-tagged sugar and the target sugar for the binding sites of surface-confined lectin and monitoring the extent of competition through highly sensitive electrochemical detection of the captured nanocrystal. This development is expected to allow decentralized detection of carbohydrate moieties and lectin-carbohydrate interactions to be performed more rapidly, sensitively, inexpensively, and reliably.

UR - https://www.scopus.com/pages/publications/33746865567

U2 - 10.1021/ja063565p

DO - 10.1021/ja063565p

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 31

ER -

Nanoparticle-based sensing of glycan-lectin interactions

Abstract

ASJC Scopus subject areas

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