Multifunctional polymer-derivatized γ-Fe2O3 nanocrystals as a methodology for the biomagnetic separation of recombinant His-tagged proteins

M. I. Shukoor, F. Natalio, M. N. Tahir, M. Divekar, N. Metz, H. A. Therese, P. Theato, V. Ksenofontov, H. C. Schröder, W. E.G. Müller, W. Tremel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Multifunctional polymer-derivatized superparamagnetic iron oxide (γ-Fe2O3) nanoparticles were prepared for biomagnetic separation of histidine-tagged recombinant proteins building up a faster and efficient method for protein separation by making use of their intrinsic magnetic properties. Using polymer bound γ-Fe2O3 nanocrystals, a 6× histidine-tagged recombinant protein (silicatein) with a molecular weight of 24 kDa has been isolated and purified. The supermagnetic iron oxide nanocrystals were characterized by transmission electron microscopy (TEM), high-resolution TEM (HRTEM), SQUID and Mössbauer and the polymer functionalization of the γ-Fe2O3 nanocrystals was monitored by UV-vis spectroscopy and light microscopy. Protein immobilization and separation was monitored using immunostaining techniques and gel electrophoresis, respectively.

Original languageEnglish
Pages (from-to)2339-2344
Number of pages6
JournalJournal of Magnetism and Magnetic Materials
Volume320
Issue number19
DOIs
StatePublished - Oct 2008
Externally publishedYes

Bibliographical note

Funding Information:
This research was supported by the Deutsche Forschungsgemeinschaft, the Bundesministerium für Bildung und Forschung, Germany [Center of Excellence BIOTECmarin], the European Society for Marine Biotechnology, the International Human Frontier Science Program, and the Materials Science Center (MWFZ) at the University of Mainz.

Keywords

  • Maghemite
  • Multifunctional nanoparticle
  • Nanoparticle
  • Protein separation
  • Silicatein
  • Surface functionalization

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

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