Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa

Edwin Ochong; Alexis Nzila; Serah Kimani; Gilbert Kokwaro; Theonest Mutabingwa; William Watkins; Kevin Marsh

doi:10.1186/1475-2875-2-1

Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa

Edwin Ochong, Alexis Nzila^*, Serah Kimani, Gilbert Kokwaro, Theonest Mutabingwa, William Watkins, Kevin Marsh

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine/pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation.

Original language	English
Article number	1
Pages (from-to)	1-3
Number of pages	3
Journal	Malaria Journal
Volume	2
DOIs	https://doi.org/10.1186/1475-2875-2-1
State	Published - 15 Dec 2003
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Infectious Diseases

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title = "Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa",

abstract = "The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine/pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation.",

author = "Edwin Ochong and Alexis Nzila and Serah Kimani and Gilbert Kokwaro and Theonest Mutabingwa and William Watkins and Kevin Marsh",

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T1 - Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa

AU - Ochong, Edwin

AU - Nzila, Alexis

AU - Kimani, Serah

AU - Kokwaro, Gilbert

AU - Mutabingwa, Theonest

AU - Watkins, William

AU - Marsh, Kevin

PY - 2003/12/15

Y1 - 2003/12/15

N2 - The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine/pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation.

AB - The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine/pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation.

UR - https://www.scopus.com/pages/publications/3042595929

U2 - 10.1186/1475-2875-2-1

DO - 10.1186/1475-2875-2-1

M3 - Article

AN - SCOPUS:3042595929

SN - 1475-2875

VL - 2

SP - 1

EP - 3

JO - Malaria Journal

JF - Malaria Journal

M1 - 1

ER -

Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa

Abstract

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