TY - JOUR
T1 - Mimicking cellular transport mechanism in stem cells through endosomal escape of new peptide-coated quantum dots
AU - Narayanan, Karthikeyan
AU - Yen, Swee Kuan
AU - Dou, Qingqing
AU - Padmanabhan, Parasuraman
AU - Sudhaharan, Thankiah
AU - Ahmed, Sohail
AU - Ying, Jackie Y.
AU - Selvan, Subramanian Tamil
PY - 2013
Y1 - 2013
N2 - Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic systems mimicking the biological protein transport mechanism. Here we report a highly efficient method of mimicking the microtubule mediated protein transport using newly designed biotinylated peptides encompassing a microtubule-associated sequence (MTAS) and a nuclear localization signaling (NLS) sequence, and their final conjugation with streptavidin-coated CdSe/ZnS quantum dots (QDs). Our results demonstrate that these novel bio-conjugated QDs enhance the endosomal escape and promote targeted delivery into the nucleus of human mesenchymal stem cells via microtubules. Mimicking the cellular transport mechanism in stem cells is highly desirable for diagnostics, targeting and therapeutic applications, opening up new avenues in the area of drug delivery.
AB - Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic systems mimicking the biological protein transport mechanism. Here we report a highly efficient method of mimicking the microtubule mediated protein transport using newly designed biotinylated peptides encompassing a microtubule-associated sequence (MTAS) and a nuclear localization signaling (NLS) sequence, and their final conjugation with streptavidin-coated CdSe/ZnS quantum dots (QDs). Our results demonstrate that these novel bio-conjugated QDs enhance the endosomal escape and promote targeted delivery into the nucleus of human mesenchymal stem cells via microtubules. Mimicking the cellular transport mechanism in stem cells is highly desirable for diagnostics, targeting and therapeutic applications, opening up new avenues in the area of drug delivery.
UR - https://www.scopus.com/pages/publications/84880367796
U2 - 10.1038/srep02184
DO - 10.1038/srep02184
M3 - Article
C2 - 23851637
AN - SCOPUS:84880367796
SN - 2045-2322
VL - 3
JO - Scientific Reports
JF - Scientific Reports
M1 - 2184
ER -