Microfluidic device with asymmetric electrodes for cell and reagent delivery

  • Daniel Lee*
  • , Guolin Xu
  • , Hong Kiat Tay
  • , Chun Yang
  • , Jackie Y. Ying
  • *Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

We present the design and fabrication of a micro-electromechanical system (MEMS) device for cell and particle delivery using a combination of AC electrokinetic fluidic flow and negative dielectrophoresis (DEP) force. An array of interdigitated asymmetric microelectrode pairs were used in the planar device. The electrodes produced a net charge in the surrounding fluid, generating an AC electrokinetic fluidic motion. A non-uniform electric field with low actuation frequency from the microelectrode pairs resulted in a negative DEP force, which was responsible for pushing delivery particles away from sedimentation. The experimental results showed that the flow velocity increased rapidly from 267 μm/min to 394 μm/min when the applied frequency was increased from 10 kHz to 70 kHz for a cell-suspending medium buffer solution with a conductivity of 4.7 μS/cm. A maximum delivery velocity of 801 μm/min was obtained when the buffer conductivity was increased to 47 μS/cm with an actuation frequency of 100 kHz.

Original languageEnglish
Title of host publicationMicro- and Nanotechnology
Subtitle of host publicationMaterials, Processes, Packaging, and Systems III
DOIs
StatePublished - 2007
Externally publishedYes
EventMicro- and Nanotechnology: Materials, Processes, Packaging, and Systems III - Adelaide, Australia
Duration: 11 Dec 200613 Dec 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6415
ISSN (Print)0277-786X

Conference

ConferenceMicro- and Nanotechnology: Materials, Processes, Packaging, and Systems III
Country/TerritoryAustralia
CityAdelaide
Period11/12/0613/12/06

Keywords

  • AC electrokinetic flow
  • Dielectrophoresis
  • Fluid and particles delivery
  • Microelectrode array

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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