Micellization, anti-proliferative activity and binding study of cationic gemini surfactants with calf thymus DNA

Farooq Ahmad Wani; Kamalakanta Behera; Rayees Ahmad Padder; Mohammad Husain; Maqsood Ahmad Malik; Nada Shaeel Al-Thabaiti; Rabia Ahmad; Rajan Patel

doi:10.1016/j.colcom.2019.100221

Micellization, anti-proliferative activity and binding study of cationic gemini surfactants with calf thymus DNA

Farooq Ahmad Wani, Kamalakanta Behera, Rayees Ahmad Padder, Mohammad Husain, Maqsood Ahmad Malik, Nada Shaeel Al-Thabaiti, Rabia Ahmad, Rajan Patel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Aggregation behaviour of two cation gemini surfactants i.e., 1,3-bis (1-decylbenzimidazolium) propane bromide BG10 and 1,3-bis (1-octylbenzimidazolium) propane bromide BG8 was studied using conductivity and dynamic light scattering (DLS). The enthalpy (∆H_m ^о), entropy (∆S_m ^о) and Gibbs free energy of micellization (∆G_m ^о) have been observed from the conductivity method at various temperatures. The anti-proliferative potential of these novel gemini surfactants against human non-small lung cancer cell line-H1299 has been investigated. Both gemini surfactants, BG8 and BG10 showed a considerable inhibition in cancer cell proliferation and IC₅₀ values are observed to reduce with chain length. The ctDNA binding was also observed by UV–visible, transmission electron microscopy (TEM), ethidium bromide (EtBr) exclusion experiments, agarose gel electrophoresis, circular dichroism (CD) and viscometry techniques. These results were further validated by in-silico studies. The binding capability of BG surfactants to ctDNA can be observed from their ability to remove EtBr from the intercalating complex of DNA resulting in fluorescence quenching.

Original language	English
Article number	100221
Journal	Colloids and Interface Science Communications
Volume	34
DOIs	https://doi.org/10.1016/j.colcom.2019.100221
State	Published - Jan 2020
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 Elsevier B.V.

Keywords

Anti-proliferative
Calf thymus-DNA
Circular dichroism
Gel electrophoresis
Gemini surfactants

ASJC Scopus subject areas

Biotechnology
Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Colloid and Surface Chemistry
Materials Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.colcom.2019.100221

Cite this

@article{0f550daa687f46c7abe7d1f55dcf83c5,

title = "Micellization, anti-proliferative activity and binding study of cationic gemini surfactants with calf thymus DNA",

abstract = "Aggregation behaviour of two cation gemini surfactants i.e., 1,3-bis (1-decylbenzimidazolium) propane bromide BG10 and 1,3-bis (1-octylbenzimidazolium) propane bromide BG8 was studied using conductivity and dynamic light scattering (DLS). The enthalpy (∆Hm о), entropy (∆Sm о) and Gibbs free energy of micellization (∆Gm о) have been observed from the conductivity method at various temperatures. The anti-proliferative potential of these novel gemini surfactants against human non-small lung cancer cell line-H1299 has been investigated. Both gemini surfactants, BG8 and BG10 showed a considerable inhibition in cancer cell proliferation and IC50 values are observed to reduce with chain length. The ctDNA binding was also observed by UV–visible, transmission electron microscopy (TEM), ethidium bromide (EtBr) exclusion experiments, agarose gel electrophoresis, circular dichroism (CD) and viscometry techniques. These results were further validated by in-silico studies. The binding capability of BG surfactants to ctDNA can be observed from their ability to remove EtBr from the intercalating complex of DNA resulting in fluorescence quenching.",

keywords = "Anti-proliferative, Calf thymus-DNA, Circular dichroism, Gel electrophoresis, Gemini surfactants",

author = "Wani, \{Farooq Ahmad\} and Kamalakanta Behera and Padder, \{Rayees Ahmad\} and Mohammad Husain and Malik, \{Maqsood Ahmad\} and Al-Thabaiti, \{Nada Shaeel\} and Rabia Ahmad and Rajan Patel",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

year = "2020",

month = jan,

doi = "10.1016/j.colcom.2019.100221",

language = "English",

volume = "34",

journal = "Colloids and Interface Science Communications",

issn = "2215-0382",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Micellization, anti-proliferative activity and binding study of cationic gemini surfactants with calf thymus DNA

AU - Wani, Farooq Ahmad

AU - Behera, Kamalakanta

AU - Padder, Rayees Ahmad

AU - Husain, Mohammad

AU - Malik, Maqsood Ahmad

AU - Al-Thabaiti, Nada Shaeel

AU - Ahmad, Rabia

AU - Patel, Rajan

PY - 2020/1

Y1 - 2020/1

N2 - Aggregation behaviour of two cation gemini surfactants i.e., 1,3-bis (1-decylbenzimidazolium) propane bromide BG10 and 1,3-bis (1-octylbenzimidazolium) propane bromide BG8 was studied using conductivity and dynamic light scattering (DLS). The enthalpy (∆Hm о), entropy (∆Sm о) and Gibbs free energy of micellization (∆Gm о) have been observed from the conductivity method at various temperatures. The anti-proliferative potential of these novel gemini surfactants against human non-small lung cancer cell line-H1299 has been investigated. Both gemini surfactants, BG8 and BG10 showed a considerable inhibition in cancer cell proliferation and IC50 values are observed to reduce with chain length. The ctDNA binding was also observed by UV–visible, transmission electron microscopy (TEM), ethidium bromide (EtBr) exclusion experiments, agarose gel electrophoresis, circular dichroism (CD) and viscometry techniques. These results were further validated by in-silico studies. The binding capability of BG surfactants to ctDNA can be observed from their ability to remove EtBr from the intercalating complex of DNA resulting in fluorescence quenching.

AB - Aggregation behaviour of two cation gemini surfactants i.e., 1,3-bis (1-decylbenzimidazolium) propane bromide BG10 and 1,3-bis (1-octylbenzimidazolium) propane bromide BG8 was studied using conductivity and dynamic light scattering (DLS). The enthalpy (∆Hm о), entropy (∆Sm о) and Gibbs free energy of micellization (∆Gm о) have been observed from the conductivity method at various temperatures. The anti-proliferative potential of these novel gemini surfactants against human non-small lung cancer cell line-H1299 has been investigated. Both gemini surfactants, BG8 and BG10 showed a considerable inhibition in cancer cell proliferation and IC50 values are observed to reduce with chain length. The ctDNA binding was also observed by UV–visible, transmission electron microscopy (TEM), ethidium bromide (EtBr) exclusion experiments, agarose gel electrophoresis, circular dichroism (CD) and viscometry techniques. These results were further validated by in-silico studies. The binding capability of BG surfactants to ctDNA can be observed from their ability to remove EtBr from the intercalating complex of DNA resulting in fluorescence quenching.

KW - Anti-proliferative

KW - Calf thymus-DNA

KW - Circular dichroism

KW - Gel electrophoresis

KW - Gemini surfactants

UR - https://www.scopus.com/pages/publications/85076528851

U2 - 10.1016/j.colcom.2019.100221

DO - 10.1016/j.colcom.2019.100221

M3 - Article

AN - SCOPUS:85076528851

SN - 2215-0382

VL - 34

JO - Colloids and Interface Science Communications

JF - Colloids and Interface Science Communications

M1 - 100221

ER -

Micellization, anti-proliferative activity and binding study of cationic gemini surfactants with calf thymus DNA

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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