Magnetoelectric core-shell CoFe2O4@BaTiO3 nanorods: their role in drug delivery and effect on multidrug resistance pump activity in vitro

Sadaf Mushtaq; Khuram Shahzad; Muhammad Rizwan; Anwar Ul-Hamid; Bilal Haider Abbasi; Waqas Khalid; Muhammad Atif; Nafees Ahmad; Zulqurnain Ali; Rashda Abbasi

doi:10.1039/d2ra03429h

Magnetoelectric core-shell CoFe₂O₄@BaTiO₃ nanorods: their role in drug delivery and effect on multidrug resistance pump activity in vitro

Sadaf Mushtaq
, Khuram Shahzad
, Muhammad Rizwan
, Anwar Ul-Hamid
, Bilal Haider Abbasi
, Waqas Khalid
, Muhammad Atif
, Nafees Ahmad
, Zulqurnain Ali
, Rashda Abbasi^*

^*Corresponding author for this work

Core Research Facilities

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Nanoparticle mediated targeted drug delivery has become a widespread area of cancer research to address premature drug delivery problems. We report the synthesis of magneto-electric (ME) core-shell cobalt ferrite-barium titanate nanorods (CFO@BTO NRs) to achieve “on demand” drug release in vitro. Physical characterizations confirmed the formation of pure CFO@BTO NRs with appropriate magnetic and ferroelectric response, favorable for an externally controlled drug delivery system. Functionalization of NRs with doxorubicin (DOX) and methotrexate (MTX) achieved up to 98% drug release in 20 minutes, under a 4 mT magnetic field (MF). We observed strong MF and dose dependent cytotoxic response in HepG2 and HT144 cells and 3D spheroid models (p < 0.05). Cytotoxicity was characterized by enhanced oxidative stress, causing p53 mediated cell cycle arrest, DNA damage and cellular apoptosis via downregulation of Bcl-2 expression. In addition, MF and dose dependent inhibition of Multidrug Resistance (MDR) pump activity was also observed (p < 0.05) indicating effectivity in chemo-resistant cancers. Hence, CFO@BTO NRs represent an efficient carrier system for controlled drug delivery in cancer nanotherapeutics, where higher drug uptake is a prerequisite for effective treatment.

Original language	English
Pages (from-to)	24958-24979
Number of pages	22
Journal	RSC Advances
Volume	12
Issue number	38
DOIs	https://doi.org/10.1039/d2ra03429h
State	Published - 1 Sep 2022

Bibliographical note

Publisher Copyright:
© 2022 The Royal Society of Chemistry.

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1039/d2ra03429h

Cite this

@article{9e53555de0c84695a421f8973be7c4c8,

title = "Magnetoelectric core-shell CoFe2O4@BaTiO3 nanorods: their role in drug delivery and effect on multidrug resistance pump activity in vitro",

abstract = "Nanoparticle mediated targeted drug delivery has become a widespread area of cancer research to address premature drug delivery problems. We report the synthesis of magneto-electric (ME) core-shell cobalt ferrite-barium titanate nanorods (CFO@BTO NRs) to achieve “on demand” drug release in vitro. Physical characterizations confirmed the formation of pure CFO@BTO NRs with appropriate magnetic and ferroelectric response, favorable for an externally controlled drug delivery system. Functionalization of NRs with doxorubicin (DOX) and methotrexate (MTX) achieved up to 98\% drug release in 20 minutes, under a 4 mT magnetic field (MF). We observed strong MF and dose dependent cytotoxic response in HepG2 and HT144 cells and 3D spheroid models (p < 0.05). Cytotoxicity was characterized by enhanced oxidative stress, causing p53 mediated cell cycle arrest, DNA damage and cellular apoptosis via downregulation of Bcl-2 expression. In addition, MF and dose dependent inhibition of Multidrug Resistance (MDR) pump activity was also observed (p < 0.05) indicating effectivity in chemo-resistant cancers. Hence, CFO@BTO NRs represent an efficient carrier system for controlled drug delivery in cancer nanotherapeutics, where higher drug uptake is a prerequisite for effective treatment.",

author = "Sadaf Mushtaq and Khuram Shahzad and Muhammad Rizwan and Anwar Ul-Hamid and Abbasi, \{Bilal Haider\} and Waqas Khalid and Muhammad Atif and Nafees Ahmad and Zulqurnain Ali and Rashda Abbasi",

note = "Publisher Copyright: {\textcopyright} 2022 The Royal Society of Chemistry.",

year = "2022",

month = sep,

day = "1",

doi = "10.1039/d2ra03429h",

language = "English",

volume = "12",

pages = "24958--24979",

journal = "RSC Advances",

issn = "2046-2069",

publisher = "Royal Society of Chemistry",

number = "38",

}

TY - JOUR

T1 - Magnetoelectric core-shell CoFe2O4@BaTiO3 nanorods

T2 - their role in drug delivery and effect on multidrug resistance pump activity in vitro

AU - Mushtaq, Sadaf

AU - Shahzad, Khuram

AU - Rizwan, Muhammad

AU - Ul-Hamid, Anwar

AU - Abbasi, Bilal Haider

AU - Khalid, Waqas

AU - Atif, Muhammad

AU - Ahmad, Nafees

AU - Ali, Zulqurnain

AU - Abbasi, Rashda

PY - 2022/9/1

Y1 - 2022/9/1

N2 - Nanoparticle mediated targeted drug delivery has become a widespread area of cancer research to address premature drug delivery problems. We report the synthesis of magneto-electric (ME) core-shell cobalt ferrite-barium titanate nanorods (CFO@BTO NRs) to achieve “on demand” drug release in vitro. Physical characterizations confirmed the formation of pure CFO@BTO NRs with appropriate magnetic and ferroelectric response, favorable for an externally controlled drug delivery system. Functionalization of NRs with doxorubicin (DOX) and methotrexate (MTX) achieved up to 98% drug release in 20 minutes, under a 4 mT magnetic field (MF). We observed strong MF and dose dependent cytotoxic response in HepG2 and HT144 cells and 3D spheroid models (p < 0.05). Cytotoxicity was characterized by enhanced oxidative stress, causing p53 mediated cell cycle arrest, DNA damage and cellular apoptosis via downregulation of Bcl-2 expression. In addition, MF and dose dependent inhibition of Multidrug Resistance (MDR) pump activity was also observed (p < 0.05) indicating effectivity in chemo-resistant cancers. Hence, CFO@BTO NRs represent an efficient carrier system for controlled drug delivery in cancer nanotherapeutics, where higher drug uptake is a prerequisite for effective treatment.

AB - Nanoparticle mediated targeted drug delivery has become a widespread area of cancer research to address premature drug delivery problems. We report the synthesis of magneto-electric (ME) core-shell cobalt ferrite-barium titanate nanorods (CFO@BTO NRs) to achieve “on demand” drug release in vitro. Physical characterizations confirmed the formation of pure CFO@BTO NRs with appropriate magnetic and ferroelectric response, favorable for an externally controlled drug delivery system. Functionalization of NRs with doxorubicin (DOX) and methotrexate (MTX) achieved up to 98% drug release in 20 minutes, under a 4 mT magnetic field (MF). We observed strong MF and dose dependent cytotoxic response in HepG2 and HT144 cells and 3D spheroid models (p < 0.05). Cytotoxicity was characterized by enhanced oxidative stress, causing p53 mediated cell cycle arrest, DNA damage and cellular apoptosis via downregulation of Bcl-2 expression. In addition, MF and dose dependent inhibition of Multidrug Resistance (MDR) pump activity was also observed (p < 0.05) indicating effectivity in chemo-resistant cancers. Hence, CFO@BTO NRs represent an efficient carrier system for controlled drug delivery in cancer nanotherapeutics, where higher drug uptake is a prerequisite for effective treatment.

UR - https://www.scopus.com/pages/publications/85138468010

U2 - 10.1039/d2ra03429h

DO - 10.1039/d2ra03429h

M3 - Article

AN - SCOPUS:85138468010

SN - 2046-2069

VL - 12

SP - 24958

EP - 24979

JO - RSC Advances

JF - RSC Advances

IS - 38

ER -