Lysinibacillus composti sp. nov., isolated from compost

A Gram-negative, motile, rod-shaped, endospore-forming bacterial strain, designated as NCCP-36^T, was isolated from the compost of fruit and vegetable wastes. The strain NCCP-36^T grew within a temperature range of 10-45∈^°C (optimum 28∈^°C) and a pH range of 6.5-8.5 (optimum 7.0), and its cells tolerated <50 mM boron (optimum growth without boron) and 0-5 % NaCl (w/v) in tryptic soya broth medium. Based on comparative analysis of 16S rRNA gene sequence, strain NCCP-36^T showed the highest similarity to Lysinibacillus sinduriensis BLB-1^T (97.52 %) and L. xylanilyticus XDB9^T (96.96 %), and <97 % similarity with other closely related taxa. However, DNA-DNA relatedness between strain NCCP-36^T and the closely related type strains of genus Lysinibacillus was ≤37 %. Phylogenetic and chemotaxonomic analyses [major polar lipids: diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phospholipids; predominant menaquinone: MK-7; major cellular fatty acids: iso-C antieso-C and iso-C DNA G+C contents: 37 mol %; Lys-Asp (type A4α) in cell-wall peptidoglycans as diagnostic amino acids] also support the affiliation of strain NCCP-36to genus Lysinibacillus. Based upon DNA-DNA relatedness as well as distinctive chemotaxonomic, phylogenetic, and genotypic data, we conclude that strain NCCP-36bsupesup belongs to a novel species of genus Lysinibacillus, for which the name Lysinibacillus composti sp. nov. is proposed. The type strain is NCCP-36bsupesup (JCM 18777 bsupesup=∈KCTC 13796 bsupesup=∈DSMZ 24785 bsupesup ).