Lipase immobilized on magnetic hierarchically porous carbon materials as a versatile tool for the synthesis of bioactive quercetin derivatives

Alexandra V. Chatzikonstantinou, Angeliki C. Polydera, Eleni Thomou, Nikolaos Chalmpes, Turki N. Baroud, Apostolos Enotiadis, Luiz Estevez, Michaela Patila, Mohamed Amen Hammami, Konstantinos Spyrou, Emmanuel P. Giannelis, Andreas G. Tzakos, Dimitrios Gournis, Haralambos Stamatis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The preparation, characterization and application of a novel robust nanobiocatalyst, developed through the covalent binding of lipase B from Pseudozyma antarctica on magnetic hierarchically porous carbon materials (HPCFe), is reported. The nanobiocatalyst was characterized by combination of spectroscopic and microscopic techniques. Structural and catalytic characterization indicates that HPCFe nanostructures create a microenvironment which stabilizes the structure of the immobilized enzyme, resulting in enhanced activity and stability in non-aqueous media over other forms of the biocatalyst. The nanobiocatalyst was effectively applied for the selective deacetylation of peracetylated quercetin towards the synthesis of 3,5,7-triacetoxy-3′,4′-dihydroxyflavone, a compound with high antiproliferative activity. At the optimum bioprocess conditions, the produced amount of the bioactive quercetin derivative in a single-step process reached values up to 3.48 g L 1 which is 13 times higher than that reported to date. The immobilized enzyme retains ~100% of its catalytic activity after 10 repeated reaction cycles.

Original languageEnglish
Article number100372
JournalBioresource Technology Reports
Volume9
DOIs
StatePublished - Feb 2020

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

  • Immobilization
  • Lipase B
  • Nanobiocatalysis
  • Porous magnetic nanomaterials
  • Quercetin acetyl analogues

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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