Integrated one-step and two-step gene synthesis on a chip

M. C. Huang; Y. K. Kuan; H. Ye; M. H. Li; J. Y. Ying

Integrated one-step and two-step gene synthesis on a chip

M. C. Huang
, Y. K. Kuan
, H. Ye
, M. H. Li
, J. Y. Ying

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper reports an integrated chip that is capable of assembling short oligonucleotides into a long DNA sequence. The chip consists of two polymerase chain reactions (PCRs), hydrogel valves, micromixer and volume meter fabricated using a fast prototyping method without lithography process. The fabricated device is combined with a miniaturized thermal cycler to perform gene synthesis.

Original language	English
Pages	423-425
Number of pages	3
State	Published - 2008
Externally published	Yes
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 12 Oct 2008 → 16 Oct 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	12/10/08 → 16/10/08

Keywords

Gene synthesis
Hydrogel valve
Rapid prototype

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Integrated one-step and two-step gene synthesis on a chip",

abstract = "This paper reports an integrated chip that is capable of assembling short oligonucleotides into a long DNA sequence. The chip consists of two polymerase chain reactions (PCRs), hydrogel valves, micromixer and volume meter fabricated using a fast prototyping method without lithography process. The fabricated device is combined with a miniaturized thermal cycler to perform gene synthesis.",

keywords = "Gene synthesis, Hydrogel valve, Rapid prototype",

author = "Huang, \{M. C.\} and Kuan, \{Y. K.\} and H. Ye and Li, \{M. H.\} and Ying, \{J. Y.\}",

year = "2008",

language = "English",

pages = "423--425",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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TY - CONF

T1 - Integrated one-step and two-step gene synthesis on a chip

AU - Huang, M. C.

AU - Kuan, Y. K.

AU - Ye, H.

AU - Li, M. H.

AU - Ying, J. Y.

PY - 2008

Y1 - 2008

N2 - This paper reports an integrated chip that is capable of assembling short oligonucleotides into a long DNA sequence. The chip consists of two polymerase chain reactions (PCRs), hydrogel valves, micromixer and volume meter fabricated using a fast prototyping method without lithography process. The fabricated device is combined with a miniaturized thermal cycler to perform gene synthesis.

AB - This paper reports an integrated chip that is capable of assembling short oligonucleotides into a long DNA sequence. The chip consists of two polymerase chain reactions (PCRs), hydrogel valves, micromixer and volume meter fabricated using a fast prototyping method without lithography process. The fabricated device is combined with a miniaturized thermal cycler to perform gene synthesis.

KW - Gene synthesis

KW - Hydrogel valve

KW - Rapid prototype

UR - https://www.scopus.com/pages/publications/84902515037

M3 - Paper

AN - SCOPUS:84902515037

SP - 423

EP - 425

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Integrated one-step and two-step gene synthesis on a chip

Abstract

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

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Cite this