Insight into the apoptosis-inducing action of α-bisabolol towards malignant tumor cells: Involvement of lipid rafts and Bid

Elena Darra; Safwat Abdel-Azeim; Anna Manara; Kazuo Shoji; Jean Didier Maréchal; Sofia Mariotto; Elisabetta Cavalieri; Luigi Perbellini; Cosimo Pizza; David Perahia; Massimo Crimi; Hisanori Suzuki

doi:10.1016/j.abb.2008.02.004

Insight into the apoptosis-inducing action of α-bisabolol towards malignant tumor cells: Involvement of lipid rafts and Bid

Elena Darra
, Safwat Abdel-Azeim
, Anna Manara
, Kazuo Shoji
, Jean Didier Maréchal
, Sofia Mariotto
, Elisabetta Cavalieri
, Luigi Perbellini
, Cosimo Pizza
, David Perahia
, Massimo Crimi
, Hisanori Suzuki^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

In a precedent report we showed that α-bisabolol, a sesquiterpene present widely in the plant kingdom, exerts a rapid and efficient apoptosis-inducing action selectively towards human and murine malignant glioblastoma cell lines through mitochondrial damage. The present study extends these data demonstrating the apoptosis-inducing action of α-bisabolol towards highly malignant human pancreatic carcinoma cell lines without affecting human fibroblast viability. The present study further shows the preferential incorporation of α-bisabolol to transformed cells through lipid rafts on plasma membranes and, thereafter, direct interaction between α-bisabolol and Bid protein, one of pro-apoptotic Bcl-2 family proteins, analyzed either by Surface Plasmon Resonance method or by intrinsic fluorescence measurement. Notions that lipid rafts are rich in plasma membranes of transformed cells and that Bid, richly present in lipid rafts, is deeply involved in lipid transport make highly credible the hypothesis that the molecular mechanism of α-bisabolol action may include its capacity to interact with Bid protein.

Original language	English
Pages (from-to)	113-123
Number of pages	11
Journal	Archives of Biochemistry and Biophysics
Volume	476
Issue number	2
DOIs	https://doi.org/10.1016/j.abb.2008.02.004
State	Published - 15 Aug 2008
Externally published	Yes

Bibliographical note

Funding Information:
Authors thank technical assistance of Francesco Pasini for GC–MS analysis. SPR was partly performed at Tokyo University of Science (Chiba, Japan) with a kind help of Prof. Tanuma. The manuscript was carefully proofed by Michelle Strauss. The present work was financially supported by Cariverona Foundation (Cariverona project 2003 to H.S.) and 60% (to H.S. and M.C.).

Keywords

Apoptosis
Bid
Lipid rafts
Mitochondria
Pancreatic carcinoma cell lines
Surface Plasmon Resonance
α-Bisabolol

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.abb.2008.02.004

Cite this

Darra, E., Abdel-Azeim, S., Manara, A., Shoji, K., Maréchal, J. D., Mariotto, S., Cavalieri, E., Perbellini, L., Pizza, C., Perahia, D., Crimi, M., & Suzuki, H. (2008). Insight into the apoptosis-inducing action of α-bisabolol towards malignant tumor cells: Involvement of lipid rafts and Bid. Archives of Biochemistry and Biophysics, 476(2), 113-123. https://doi.org/10.1016/j.abb.2008.02.004

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title = "Insight into the apoptosis-inducing action of α-bisabolol towards malignant tumor cells: Involvement of lipid rafts and Bid",

abstract = "In a precedent report we showed that α-bisabolol, a sesquiterpene present widely in the plant kingdom, exerts a rapid and efficient apoptosis-inducing action selectively towards human and murine malignant glioblastoma cell lines through mitochondrial damage. The present study extends these data demonstrating the apoptosis-inducing action of α-bisabolol towards highly malignant human pancreatic carcinoma cell lines without affecting human fibroblast viability. The present study further shows the preferential incorporation of α-bisabolol to transformed cells through lipid rafts on plasma membranes and, thereafter, direct interaction between α-bisabolol and Bid protein, one of pro-apoptotic Bcl-2 family proteins, analyzed either by Surface Plasmon Resonance method or by intrinsic fluorescence measurement. Notions that lipid rafts are rich in plasma membranes of transformed cells and that Bid, richly present in lipid rafts, is deeply involved in lipid transport make highly credible the hypothesis that the molecular mechanism of α-bisabolol action may include its capacity to interact with Bid protein.",

keywords = "Apoptosis, Bid, Lipid rafts, Mitochondria, Pancreatic carcinoma cell lines, Surface Plasmon Resonance, α-Bisabolol",

author = "Elena Darra and Safwat Abdel-Azeim and Anna Manara and Kazuo Shoji and Mar{\'e}chal, \{Jean Didier\} and Sofia Mariotto and Elisabetta Cavalieri and Luigi Perbellini and Cosimo Pizza and David Perahia and Massimo Crimi and Hisanori Suzuki",

note = "Funding Information: Authors thank technical assistance of Francesco Pasini for GC–MS analysis. SPR was partly performed at Tokyo University of Science (Chiba, Japan) with a kind help of Prof. Tanuma. The manuscript was carefully proofed by Michelle Strauss. The present work was financially supported by Cariverona Foundation (Cariverona project 2003 to H.S.) and 60\% (to H.S. and M.C.). ",

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doi = "10.1016/j.abb.2008.02.004",

language = "English",

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Darra, E, Abdel-Azeim, S, Manara, A, Shoji, K, Maréchal, JD, Mariotto, S, Cavalieri, E, Perbellini, L, Pizza, C, Perahia, D, Crimi, M & Suzuki, H 2008, 'Insight into the apoptosis-inducing action of α-bisabolol towards malignant tumor cells: Involvement of lipid rafts and Bid', Archives of Biochemistry and Biophysics, vol. 476, no. 2, pp. 113-123. https://doi.org/10.1016/j.abb.2008.02.004

TY - JOUR

T1 - Insight into the apoptosis-inducing action of α-bisabolol towards malignant tumor cells

T2 - Involvement of lipid rafts and Bid

AU - Darra, Elena

AU - Abdel-Azeim, Safwat

AU - Manara, Anna

AU - Shoji, Kazuo

AU - Maréchal, Jean Didier

AU - Mariotto, Sofia

AU - Cavalieri, Elisabetta

AU - Perbellini, Luigi

AU - Pizza, Cosimo

AU - Perahia, David

AU - Crimi, Massimo

AU - Suzuki, Hisanori

N1 - Funding Information: Authors thank technical assistance of Francesco Pasini for GC–MS analysis. SPR was partly performed at Tokyo University of Science (Chiba, Japan) with a kind help of Prof. Tanuma. The manuscript was carefully proofed by Michelle Strauss. The present work was financially supported by Cariverona Foundation (Cariverona project 2003 to H.S.) and 60% (to H.S. and M.C.).

PY - 2008/8/15

Y1 - 2008/8/15

N2 - In a precedent report we showed that α-bisabolol, a sesquiterpene present widely in the plant kingdom, exerts a rapid and efficient apoptosis-inducing action selectively towards human and murine malignant glioblastoma cell lines through mitochondrial damage. The present study extends these data demonstrating the apoptosis-inducing action of α-bisabolol towards highly malignant human pancreatic carcinoma cell lines without affecting human fibroblast viability. The present study further shows the preferential incorporation of α-bisabolol to transformed cells through lipid rafts on plasma membranes and, thereafter, direct interaction between α-bisabolol and Bid protein, one of pro-apoptotic Bcl-2 family proteins, analyzed either by Surface Plasmon Resonance method or by intrinsic fluorescence measurement. Notions that lipid rafts are rich in plasma membranes of transformed cells and that Bid, richly present in lipid rafts, is deeply involved in lipid transport make highly credible the hypothesis that the molecular mechanism of α-bisabolol action may include its capacity to interact with Bid protein.

AB - In a precedent report we showed that α-bisabolol, a sesquiterpene present widely in the plant kingdom, exerts a rapid and efficient apoptosis-inducing action selectively towards human and murine malignant glioblastoma cell lines through mitochondrial damage. The present study extends these data demonstrating the apoptosis-inducing action of α-bisabolol towards highly malignant human pancreatic carcinoma cell lines without affecting human fibroblast viability. The present study further shows the preferential incorporation of α-bisabolol to transformed cells through lipid rafts on plasma membranes and, thereafter, direct interaction between α-bisabolol and Bid protein, one of pro-apoptotic Bcl-2 family proteins, analyzed either by Surface Plasmon Resonance method or by intrinsic fluorescence measurement. Notions that lipid rafts are rich in plasma membranes of transformed cells and that Bid, richly present in lipid rafts, is deeply involved in lipid transport make highly credible the hypothesis that the molecular mechanism of α-bisabolol action may include its capacity to interact with Bid protein.

KW - Apoptosis

KW - Bid

KW - Lipid rafts

KW - Mitochondria

KW - Pancreatic carcinoma cell lines

KW - Surface Plasmon Resonance

KW - α-Bisabolol

UR - https://www.scopus.com/pages/publications/48149111801

U2 - 10.1016/j.abb.2008.02.004

DO - 10.1016/j.abb.2008.02.004

M3 - Article

C2 - 18291090

AN - SCOPUS:48149111801

SN - 0003-9861

VL - 476

SP - 113

EP - 123

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

IS - 2

ER -

Insight into the apoptosis-inducing action of α-bisabolol towards malignant tumor cells: Involvement of lipid rafts and Bid

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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Cite this