Infrared Spectroscopy for Rapid Triage of Cancer Using Blood Derivatives: A Reality Check

Shaiju S. Nazeer*, Ravi Kumar Venkataraman, Ramapurath S. Jayasree, Jagadeesh Bayry

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review


Infrared (IR) spectroscopy of serum/plasma represents an alluring molecular diagnostic tool, especially for cancer, as it can provide a molecular fingerprint of clinical samples based on vibrational modes of chemical bonds. However, despite the superior performance, the routine adoption of this technique for clinical settings has remained elusive. This is due to the potential confounding factors that are often overlooked and pose a significant barrier to clinical translation. In this Perspective, we summarize the concerns associated with various confounding factors, such as fluid sampling, optical effects, hemolysis, abnormal cardiovascular and/or hepatic functions, infections, alcoholism, diet style, age, and gender of a patient or normal control cohort, and improper selection of numerical methods that ultimately would lead to improper spectral diagnosis. We also propose some precautionary measures to overcome the challenges associated with these confounding factors.

Original languageEnglish
Pages (from-to)957-965
Number of pages9
JournalAnalytical Chemistry
Issue number3
StatePublished - 23 Jan 2024

Bibliographical note

Publisher Copyright:
© 2024 American Chemical Society

ASJC Scopus subject areas

  • Analytical Chemistry


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