High performance liquid chromatography method for determination of methyl-5-benzoyl-2-benzimidazole carbamate (mebendazole) and its main degradation product in pharmaceutical dosage forms

  • Z. Al-Kurdi
  • , T. Al-Jallad
  • , A. Badwan
  • , A. M.Y. Jaber*
  • *Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Methyl-5-benzoyl-2-benzimidazole carbamate (mebendazole) is a drug used as an anthelmintic. A high performance liquid chromatography method has been developed in this study to determine mebendazole and its degradation product in the pharmaceutical dosage forms (tablets and suspension). The expected major degradation product of mebendazole in the dosage forms has been prepared, and identified as 2-amino-5-benzoylbenzimidazole. The proposed HPLC assay was found to be selective, accurate (% recoveries were in the range of 99.9-100.9) for both, mebendazole and the degradation product, repeatable and reproducible (replicate measurements for short and long term measurements showed % RSD of ≤1.4). The methodology could be considered as a stability indicating method for mebendazole in pharmaceutical dosage forms.

Original languageEnglish
Pages (from-to)1089-1097
Number of pages9
JournalTalanta
Volume50
Issue number5
DOIs
StatePublished - Dec 1999

Bibliographical note

Funding Information:
JPM and KFUPM are thanked for the support of this research project.

Keywords

  • Degradation
  • HPLC
  • Mebendazole
  • Pharmaceutical dosage forms

ASJC Scopus subject areas

  • Analytical Chemistry

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