Factors influencing the adsorptive stripping potentiometric response of synthetic oligonucleotides

Joseph Wang; Abdel Nasser Kawde; Eskil Sahlin; Conception Parrado; Gustavo Rivas

doi:10.1002/1521-4109(200008)12:12<917::AID-ELAN917>3.0.CO;2-F

Factors influencing the adsorptive stripping potentiometric response of synthetic oligonucleotides

Joseph Wang^*, Abdel Nasser Kawde, Eskil Sahlin, Conception Parrado, Gustavo Rivas

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The adsorptive stripping potentiometric behavior of synthetic oligonucleotides at carbon paste electrodes is examined using oligo(dG)₇, oligo(dG)₁₂, oligo(dG)₁₅, oligo(dG)₂₀, oligo(dG)₃₀, and oligo(dAG)₆, and oligo(dA)₆(dG)₆ as models. Factors influencing the adsorption behavior and the resulting response are investigated. These include the role of the oligonucleotide length and concentration, its guanine +adenine content and sequence, and accumulation time. The results indicate that the stripping performance is influenced not only by the length of the oligonucleotide (i.e., degree of surface contact) but also by its base content and sequence. Trace (mg/L) levels of the oligonucleotides can thus be readily detected following short accumulation times, with good reproducibility (RSD = 3-6%) and detection limits down to 3μg/L.

Original language	English
Pages (from-to)	917-920
Number of pages	4
Journal	Electroanalysis
Volume	12
Issue number	12
DOIs	https://doi.org/10.1002/1521-4109(200008)12:12<917::AID-ELAN917>3.0.CO;2-F
State	Published - 2000
Externally published	Yes

Keywords

Adsorption
Nucleic acids
Oligonucleotides
Stripping potentiometry

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

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10.1002/1521-4109(200008)12:12<917::AID-ELAN917>3.0.CO;2-F

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title = "Factors influencing the adsorptive stripping potentiometric response of synthetic oligonucleotides",

abstract = "The adsorptive stripping potentiometric behavior of synthetic oligonucleotides at carbon paste electrodes is examined using oligo(dG)7, oligo(dG)12, oligo(dG)15, oligo(dG)20, oligo(dG)30, and oligo(dAG)6, and oligo(dA)6(dG)6 as models. Factors influencing the adsorption behavior and the resulting response are investigated. These include the role of the oligonucleotide length and concentration, its guanine +adenine content and sequence, and accumulation time. The results indicate that the stripping performance is influenced not only by the length of the oligonucleotide (i.e., degree of surface contact) but also by its base content and sequence. Trace (mg/L) levels of the oligonucleotides can thus be readily detected following short accumulation times, with good reproducibility (RSD = 3-6\%) and detection limits down to 3μg/L.",

keywords = "Adsorption, Nucleic acids, Oligonucleotides, Stripping potentiometry",

author = "Joseph Wang and Kawde, \{Abdel Nasser\} and Eskil Sahlin and Conception Parrado and Gustavo Rivas",

year = "2000",

doi = "10.1002/1521-4109(200008)12:12<917::AID-ELAN917>3.0.CO;2-F",

language = "English",

volume = "12",

pages = "917--920",

journal = "Electroanalysis",

issn = "1040-0397",

publisher = "Wiley-VCH Verlag",

number = "12",

}

TY - JOUR

T1 - Factors influencing the adsorptive stripping potentiometric response of synthetic oligonucleotides

AU - Wang, Joseph

AU - Kawde, Abdel Nasser

AU - Sahlin, Eskil

AU - Parrado, Conception

AU - Rivas, Gustavo

PY - 2000

Y1 - 2000

N2 - The adsorptive stripping potentiometric behavior of synthetic oligonucleotides at carbon paste electrodes is examined using oligo(dG)7, oligo(dG)12, oligo(dG)15, oligo(dG)20, oligo(dG)30, and oligo(dAG)6, and oligo(dA)6(dG)6 as models. Factors influencing the adsorption behavior and the resulting response are investigated. These include the role of the oligonucleotide length and concentration, its guanine +adenine content and sequence, and accumulation time. The results indicate that the stripping performance is influenced not only by the length of the oligonucleotide (i.e., degree of surface contact) but also by its base content and sequence. Trace (mg/L) levels of the oligonucleotides can thus be readily detected following short accumulation times, with good reproducibility (RSD = 3-6%) and detection limits down to 3μg/L.

AB - The adsorptive stripping potentiometric behavior of synthetic oligonucleotides at carbon paste electrodes is examined using oligo(dG)7, oligo(dG)12, oligo(dG)15, oligo(dG)20, oligo(dG)30, and oligo(dAG)6, and oligo(dA)6(dG)6 as models. Factors influencing the adsorption behavior and the resulting response are investigated. These include the role of the oligonucleotide length and concentration, its guanine +adenine content and sequence, and accumulation time. The results indicate that the stripping performance is influenced not only by the length of the oligonucleotide (i.e., degree of surface contact) but also by its base content and sequence. Trace (mg/L) levels of the oligonucleotides can thus be readily detected following short accumulation times, with good reproducibility (RSD = 3-6%) and detection limits down to 3μg/L.

KW - Adsorption

KW - Nucleic acids

KW - Oligonucleotides

KW - Stripping potentiometry

UR - https://www.scopus.com/pages/publications/0033673927

U2 - 10.1002/1521-4109(200008)12:12<917::AID-ELAN917>3.0.CO;2-F

DO - 10.1002/1521-4109(200008)12:12<917::AID-ELAN917>3.0.CO;2-F

M3 - Article

AN - SCOPUS:0033673927

SN - 1040-0397

VL - 12

SP - 917

EP - 920

JO - Electroanalysis

JF - Electroanalysis

IS - 12

ER -

Factors influencing the adsorptive stripping potentiometric response of synthetic oligonucleotides

Abstract

Keywords

ASJC Scopus subject areas

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