Fabrication of a Co-Ni/TiO2 Core-Shell Sensor for Label-Free Quantification of Human Serum Albumin in Hepatitis C Virus Patients

Noor Sultan, Ayub Alam, Batool Fatima*, Sameera Shafi, Dilshad Hussain, Zohaib Sarwar, Muhammad Wasim, Saadat Majeed, Ahmad Yar, Muhammad Najam-Ul-Haq

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Liver cirrhosis is a life-threatening disorder characterized by severe hepatitis C. Early detection of hepatitis C is crucial since HCV infection has few symptoms, and the patient may remain unaware for many years. The early-stage detection can assist in the treatment and prevent further virus transmission. Abnormally varying albumin concentrations in hepatitis C patients can lead to hypoalbuminemia. Herein, a novel Co-Ni/TiO2 core-shell-based electrochemical sensor is developed for facile and ultra-sensitive human serum albumin (HSA) detection in HCV patients. Co-Ni/TiO2 core shells are fabricated via coprecipitation and hydrothermal methods and used for non-enzymatic detection albumin with high sensitivity, selectivity, and lower detection limits. Cyclic voltammetry, chronoamperometry (CA), and electrochemical impedance spectroscopy (EIS) is performed for albumin detection via Co-Ni/TiO2 modified GCE at different scan rates, pH, and concentrations. The linear range and limit of detection (LOD) obtained for the Co-Ni/TiO2 electrode are 0-30 μM and 0.019 μM, respectively. The utility of Co-Ni/TiO2 electrodes is extended to HSA sensing from HCV-infected patients. Albumin recovery at room temperature ranged from 81.0% to 91.3% in diluted human serum samples. The developed electrochemical analysis method is superior for HSA detection and can be a tool for clinical diagnosis in the future.

Original languageEnglish
Article number097508
JournalJournal of the Electrochemical Society
Volume170
Issue number9
DOIs
StatePublished - 2023

Bibliographical note

Publisher Copyright:
© 2023 The Electrochemical Society (“ECS”). Published on behalf of ECS by IOP Publishing Limited.

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Renewable Energy, Sustainability and the Environment
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Electrochemistry
  • Materials Chemistry

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