Exploring bacterial diversity in Arctic fjord sediments: a 16S rRNA–based metabarcoding portrait

Bhavya Kachiprath, Solly Solomon, Jayanath Gopi, P. R. Jayachandran, Jabir Thajudeen, Manomi Sarasan, Anjali S. Mohan, Jayesh Puthumana, E. R. Chaithanya, Rosamma Philip*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The frosty polar environment houses diverse habitats mostly driven by psychrophilic and psychrotolerant microbes. Along with traditional cultivation methods, next-generation sequencing technologies have become common for exploring microbial communities from various extreme environments. Investigations on glaciers, ice sheets, ponds, lakes, etc. have revealed the existence of numerous microorganisms while details of microbial communities in the Arctic fjords remain incomplete. The current study focuses on understanding the bacterial diversity in two Arctic fjord sediments employing the 16S rRNA gene metabarcoding and its comparison with previous studies from various Arctic habitats. The study revealed that Proteobacteria was the dominant phylum from both the fjord samples followed by Bacteroidetes, Planctomycetes, Firmicutes, Actinobacteria, Cyanobacteria, Chloroflexi and Chlamydiae. A significant proportion of unclassified reads derived from bacteria was also detected. Psychrobacter, Pseudomonas, Acinetobacter, Aeromonas, Photobacterium, Flavobacterium, Gramella and Shewanella were the major genera in both the fjord sediments. The above findings were confirmed by the comparative analysis of fjord metadata with the previously reported (secondary metadata) Arctic samples. This study demonstrated the potential of 16S rRNA gene metabarcoding in resolving bacterial composition and diversity thereby providing new in situ insights into Arctic fjord systems.

Original languageEnglish
Pages (from-to)499-513
Number of pages15
JournalBrazilian Journal of Microbiology
Volume55
Issue number1
DOIs
StatePublished - Mar 2024

Bibliographical note

Publisher Copyright:
© The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia 2024.

Keywords

  • Arctic fjords
  • Kongsfjorden
  • Krossfjorden
  • MG-RAST
  • metabarcoding

ASJC Scopus subject areas

  • Microbiology

Fingerprint

Dive into the research topics of 'Exploring bacterial diversity in Arctic fjord sediments: a 16S rRNA–based metabarcoding portrait'. Together they form a unique fingerprint.

Cite this