Elucidating drug resistance properties in scarce cancer stem cells using droplet microarray

  • Yong Yeow Lee
  • , Karthikeyan Narayanan
  • , Shu Jun Gao
  • , Jackie Y. Ying*
  • *Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The screening and characterization of cancer stem cells (CSCs) has been challenging due to sample scarcity. We have developed a miniaturized high-content screening (HCS) assay platform to study intra-cellular proteins related to the survival and apoptosis of CSCs with chemotherapeutic treatment. By overcoming critical bottlenecks associated with sample scarcity, we are able to elucidate the drug responses of the side-population (SP) phenotype characteristics of CSCs. This successful miniaturization of HCS assays facilitates the development of cancer drug treatments, and paves the way for nanoliter volume manipulation for cell-based studies and screening.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalNano Today
Volume7
Issue number1
DOIs
StatePublished - Feb 2012
Externally publishedYes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • Cancer stem cells
  • Drug resistance
  • High-content screening

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • General Materials Science
  • Pharmaceutical Science

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