Electrospun polylactic acid/date palm polyphenol extract nanofibres for tissue engineering applications

Khadija M. Zadeh; Adriaan S. Luyt; Lubna Zarif; Robin Augustine; Anwarul Hasan; Massimo Messori; Mohammad K. Hassan; Huseyin C. Yalcin

doi:10.1007/s42247-019-00042-8

Electrospun polylactic acid/date palm polyphenol extract nanofibres for tissue engineering applications

Khadija M. Zadeh, Adriaan S. Luyt, Lubna Zarif, Robin Augustine, Anwarul Hasan, Massimo Messori, Mohammad K. Hassan^*, Huseyin C. Yalcin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

In this study, a set of polylactic acid (PLA)/polyphenol extracted from date palm fruit (DP) blends were prepared by electrospinning process to be used as cell culture scaffolds for tissue engineering applications. For this purpose, PLA/DP blends with variable composition were dissolved in dichloromethane/dimethylformamide (70:30, v/v) mixture and then electrospun to obtain the fibres. Contact angle measurements, dynamic mechanical analysis, mechanical tensile and scanning electron microscopy (SEM) tools were used to study the physico-mechanical properties of the electrospun scaffolds. The results revealed that scaffolds became more hydrophilic with addition of DP. Increasing the polyphenol concentration caused the tensile strength and Young’s modulus to decrease. The SEM graphs indicated a decrease in fibre diameter with increasing DP content. In addition, it was found that both cell proliferation and cell viability were enhanced with increased DP concentration within the scaffolds. The scratch test shows that there is an enhancement in cell migration through the scratch for PLA/DP scaffolds; again, higher DP content resulted better migration. Our results suggest that improved mechanical properties, decreased fibre diameter and enhanced hydrophilicity with addition of DP improved cell migration and cell adhesion for the scaffolds. Overall, these results demonstrate that DP is a potential natural cell-friendly product for tissue engineering applications such as tissue regeneration or wound healing assays.

Original language	English
Pages (from-to)	141-151
Number of pages	11
Journal	Emergent Materials
Volume	2
Issue number	2
DOIs	https://doi.org/10.1007/s42247-019-00042-8
State	Published - 1 Jun 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019, The Author(s).

Keywords

Cell adhesion
Date palm fruit
Electrospinning
Polylactic acid/polyphenol blend
Scaffold
Tissue engineering

ASJC Scopus subject areas

Ceramics and Composites
Biomaterials
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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10.1007/s42247-019-00042-8

Cite this

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title = "Electrospun polylactic acid/date palm polyphenol extract nanofibres for tissue engineering applications",

abstract = "In this study, a set of polylactic acid (PLA)/polyphenol extracted from date palm fruit (DP) blends were prepared by electrospinning process to be used as cell culture scaffolds for tissue engineering applications. For this purpose, PLA/DP blends with variable composition were dissolved in dichloromethane/dimethylformamide (70:30, v/v) mixture and then electrospun to obtain the fibres. Contact angle measurements, dynamic mechanical analysis, mechanical tensile and scanning electron microscopy (SEM) tools were used to study the physico-mechanical properties of the electrospun scaffolds. The results revealed that scaffolds became more hydrophilic with addition of DP. Increasing the polyphenol concentration caused the tensile strength and Young{\textquoteright}s modulus to decrease. The SEM graphs indicated a decrease in fibre diameter with increasing DP content. In addition, it was found that both cell proliferation and cell viability were enhanced with increased DP concentration within the scaffolds. The scratch test shows that there is an enhancement in cell migration through the scratch for PLA/DP scaffolds; again, higher DP content resulted better migration. Our results suggest that improved mechanical properties, decreased fibre diameter and enhanced hydrophilicity with addition of DP improved cell migration and cell adhesion for the scaffolds. Overall, these results demonstrate that DP is a potential natural cell-friendly product for tissue engineering applications such as tissue regeneration or wound healing assays.",

keywords = "Cell adhesion, Date palm fruit, Electrospinning, Polylactic acid/polyphenol blend, Scaffold, Tissue engineering",

author = "Zadeh, \{Khadija M.\} and Luyt, \{Adriaan S.\} and Lubna Zarif and Robin Augustine and Anwarul Hasan and Massimo Messori and Hassan, \{Mohammad K.\} and Yalcin, \{Huseyin C.\}",

note = "Publisher Copyright: {\textcopyright} 2019, The Author(s).",

year = "2019",

month = jun,

day = "1",

doi = "10.1007/s42247-019-00042-8",

language = "English",

volume = "2",

pages = "141--151",

journal = "Emergent Materials",

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TY - JOUR

T1 - Electrospun polylactic acid/date palm polyphenol extract nanofibres for tissue engineering applications

AU - Zadeh, Khadija M.

AU - Luyt, Adriaan S.

AU - Zarif, Lubna

AU - Augustine, Robin

AU - Hasan, Anwarul

AU - Messori, Massimo

AU - Hassan, Mohammad K.

AU - Yalcin, Huseyin C.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - In this study, a set of polylactic acid (PLA)/polyphenol extracted from date palm fruit (DP) blends were prepared by electrospinning process to be used as cell culture scaffolds for tissue engineering applications. For this purpose, PLA/DP blends with variable composition were dissolved in dichloromethane/dimethylformamide (70:30, v/v) mixture and then electrospun to obtain the fibres. Contact angle measurements, dynamic mechanical analysis, mechanical tensile and scanning electron microscopy (SEM) tools were used to study the physico-mechanical properties of the electrospun scaffolds. The results revealed that scaffolds became more hydrophilic with addition of DP. Increasing the polyphenol concentration caused the tensile strength and Young’s modulus to decrease. The SEM graphs indicated a decrease in fibre diameter with increasing DP content. In addition, it was found that both cell proliferation and cell viability were enhanced with increased DP concentration within the scaffolds. The scratch test shows that there is an enhancement in cell migration through the scratch for PLA/DP scaffolds; again, higher DP content resulted better migration. Our results suggest that improved mechanical properties, decreased fibre diameter and enhanced hydrophilicity with addition of DP improved cell migration and cell adhesion for the scaffolds. Overall, these results demonstrate that DP is a potential natural cell-friendly product for tissue engineering applications such as tissue regeneration or wound healing assays.

AB - In this study, a set of polylactic acid (PLA)/polyphenol extracted from date palm fruit (DP) blends were prepared by electrospinning process to be used as cell culture scaffolds for tissue engineering applications. For this purpose, PLA/DP blends with variable composition were dissolved in dichloromethane/dimethylformamide (70:30, v/v) mixture and then electrospun to obtain the fibres. Contact angle measurements, dynamic mechanical analysis, mechanical tensile and scanning electron microscopy (SEM) tools were used to study the physico-mechanical properties of the electrospun scaffolds. The results revealed that scaffolds became more hydrophilic with addition of DP. Increasing the polyphenol concentration caused the tensile strength and Young’s modulus to decrease. The SEM graphs indicated a decrease in fibre diameter with increasing DP content. In addition, it was found that both cell proliferation and cell viability were enhanced with increased DP concentration within the scaffolds. The scratch test shows that there is an enhancement in cell migration through the scratch for PLA/DP scaffolds; again, higher DP content resulted better migration. Our results suggest that improved mechanical properties, decreased fibre diameter and enhanced hydrophilicity with addition of DP improved cell migration and cell adhesion for the scaffolds. Overall, these results demonstrate that DP is a potential natural cell-friendly product for tissue engineering applications such as tissue regeneration or wound healing assays.

KW - Cell adhesion

KW - Date palm fruit

KW - Electrospinning

KW - Polylactic acid/polyphenol blend

KW - Scaffold

KW - Tissue engineering

UR - https://www.scopus.com/pages/publications/85075675059

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DO - 10.1007/s42247-019-00042-8

M3 - Article

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SP - 141

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JO - Emergent Materials

JF - Emergent Materials

IS - 2

ER -

Electrospun polylactic acid/date palm polyphenol extract nanofibres for tissue engineering applications

Abstract

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Keywords

ASJC Scopus subject areas

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