Electroenhanced solid-phase microextraction of methamphetamine with commercial fibers

Tsze Yin Tan; Chanbasha Basheer; Melgious Jin Yan Ang; Hian Kee Lee

doi:10.1016/j.chroma.2013.04.082

Electroenhanced solid-phase microextraction of methamphetamine with commercial fibers

Tsze Yin Tan
, Chanbasha Basheer
, Melgious Jin Yan Ang
, Hian Kee Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Electroenhanced solid-phase microextraction (EE-SPME) method with gas chromatographic mass spectrometric analysis was investigated for the determination of methamphetamine in urine sample with commercial fibers. In this approach, commercial SPME fibers were used in direct immersion mode with an applied potential to extract methamphetamine. EE-SPME was more effective in the extraction compared to conventional SPME (i.e. application of potential). The method was simple to use, and avoided the need for alkalization and derivatization of methamphetamine. Experimental conditions were optimized to achieve better extraction performance. Various conditions including applied potential, sample pH, extraction and desorption time were investigated. Based on the optimized conditions, EE-SPME achieved a higher enrichment factor of 159-fold than conventional SPME. The calibration plot under the best selected parameters was linear in the range of 0.5-15. ng/mL (r=. 0.9948). The feasibility of EE-SPME was demonstrated by applying it to the analysis of human urine samples. The limit of detection of methamphetamine was 0.25. ng/mL with a satisfactory relative standard deviation of 6.12% (n=. 3) in human urine.

Original language	English
Pages (from-to)	12-16
Number of pages	5
Journal	Journal of Chromatography A
Volume	1297
DOIs	https://doi.org/10.1016/j.chroma.2013.04.082
State	Published - 5 Jul 2013

Bibliographical note

Funding Information:
The authors are grateful to the Health Sciences Authority of Singapore for donating methamphetamine. This research was partially supported by the National Research Foundation Singapore (NRF) under its Environmental & Water Technologies Strategic Research Programme and administered by the Environment & Water Industry Programme Office (EWI) of PUB Singapore. The authors also acknowledge the support of the National University of Singapore . CB gratefully acknowledges the funding support of the King Abdul Aziz City for Science and Technology through the Science and Technology Unit at King Fahd University of Petroleum and Minerals for funding (project no. 10-WAT1396-04 ) as part of the National Science Technology and Innovation Plan.

Keywords

Electroenhanced extraction
Gas chromatography mass spectrometer
Methamphetamine

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2013.04.082

Cite this

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title = "Electroenhanced solid-phase microextraction of methamphetamine with commercial fibers",

abstract = "Electroenhanced solid-phase microextraction (EE-SPME) method with gas chromatographic mass spectrometric analysis was investigated for the determination of methamphetamine in urine sample with commercial fibers. In this approach, commercial SPME fibers were used in direct immersion mode with an applied potential to extract methamphetamine. EE-SPME was more effective in the extraction compared to conventional SPME (i.e. application of potential). The method was simple to use, and avoided the need for alkalization and derivatization of methamphetamine. Experimental conditions were optimized to achieve better extraction performance. Various conditions including applied potential, sample pH, extraction and desorption time were investigated. Based on the optimized conditions, EE-SPME achieved a higher enrichment factor of 159-fold than conventional SPME. The calibration plot under the best selected parameters was linear in the range of 0.5-15. ng/mL (r=. 0.9948). The feasibility of EE-SPME was demonstrated by applying it to the analysis of human urine samples. The limit of detection of methamphetamine was 0.25. ng/mL with a satisfactory relative standard deviation of 6.12\% (n=. 3) in human urine.",

keywords = "Electroenhanced extraction, Gas chromatography mass spectrometer, Methamphetamine",

author = "Tan, \{Tsze Yin\} and Chanbasha Basheer and \{Yan Ang\}, \{Melgious Jin\} and Lee, \{Hian Kee\}",

note = "Funding Information: The authors are grateful to the Health Sciences Authority of Singapore for donating methamphetamine. This research was partially supported by the National Research Foundation Singapore (NRF) under its Environmental \& Water Technologies Strategic Research Programme and administered by the Environment \& Water Industry Programme Office (EWI) of PUB Singapore. The authors also acknowledge the support of the National University of Singapore . CB gratefully acknowledges the funding support of the King Abdul Aziz City for Science and Technology through the Science and Technology Unit at King Fahd University of Petroleum and Minerals for funding (project no. 10-WAT1396-04 ) as part of the National Science Technology and Innovation Plan.",

year = "2013",

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doi = "10.1016/j.chroma.2013.04.082",

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AU - Tan, Tsze Yin

AU - Basheer, Chanbasha

AU - Yan Ang, Melgious Jin

AU - Lee, Hian Kee

N1 - Funding Information: The authors are grateful to the Health Sciences Authority of Singapore for donating methamphetamine. This research was partially supported by the National Research Foundation Singapore (NRF) under its Environmental & Water Technologies Strategic Research Programme and administered by the Environment & Water Industry Programme Office (EWI) of PUB Singapore. The authors also acknowledge the support of the National University of Singapore . CB gratefully acknowledges the funding support of the King Abdul Aziz City for Science and Technology through the Science and Technology Unit at King Fahd University of Petroleum and Minerals for funding (project no. 10-WAT1396-04 ) as part of the National Science Technology and Innovation Plan.

PY - 2013/7/5

Y1 - 2013/7/5

N2 - Electroenhanced solid-phase microextraction (EE-SPME) method with gas chromatographic mass spectrometric analysis was investigated for the determination of methamphetamine in urine sample with commercial fibers. In this approach, commercial SPME fibers were used in direct immersion mode with an applied potential to extract methamphetamine. EE-SPME was more effective in the extraction compared to conventional SPME (i.e. application of potential). The method was simple to use, and avoided the need for alkalization and derivatization of methamphetamine. Experimental conditions were optimized to achieve better extraction performance. Various conditions including applied potential, sample pH, extraction and desorption time were investigated. Based on the optimized conditions, EE-SPME achieved a higher enrichment factor of 159-fold than conventional SPME. The calibration plot under the best selected parameters was linear in the range of 0.5-15. ng/mL (r=. 0.9948). The feasibility of EE-SPME was demonstrated by applying it to the analysis of human urine samples. The limit of detection of methamphetamine was 0.25. ng/mL with a satisfactory relative standard deviation of 6.12% (n=. 3) in human urine.

AB - Electroenhanced solid-phase microextraction (EE-SPME) method with gas chromatographic mass spectrometric analysis was investigated for the determination of methamphetamine in urine sample with commercial fibers. In this approach, commercial SPME fibers were used in direct immersion mode with an applied potential to extract methamphetamine. EE-SPME was more effective in the extraction compared to conventional SPME (i.e. application of potential). The method was simple to use, and avoided the need for alkalization and derivatization of methamphetamine. Experimental conditions were optimized to achieve better extraction performance. Various conditions including applied potential, sample pH, extraction and desorption time were investigated. Based on the optimized conditions, EE-SPME achieved a higher enrichment factor of 159-fold than conventional SPME. The calibration plot under the best selected parameters was linear in the range of 0.5-15. ng/mL (r=. 0.9948). The feasibility of EE-SPME was demonstrated by applying it to the analysis of human urine samples. The limit of detection of methamphetamine was 0.25. ng/mL with a satisfactory relative standard deviation of 6.12% (n=. 3) in human urine.

KW - Electroenhanced extraction

KW - Gas chromatography mass spectrometer

KW - Methamphetamine

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Electroenhanced solid-phase microextraction of methamphetamine with commercial fibers

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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