Effects of annealing temperature on crystal structure and glucose sensing properties of cuprous oxide

The diabetes mellitus was reported as one of the leading reasons for death around the world. Consequently, most of the researches were ardent to the detection of blood sugar level. Therefore, the morphology, as well as the sensing properties of renowned materials, should have optimized and engineered for higher sensitivity towards glucose. For the first time, an extensively utilized active component of a glucose sensor, cuprous oxide (Cu₂O) is synthesized and dealt with various annealing temperatures at 400, 600, and 800 °C. The impacts of annealing temperatures on morphology, electro-active surface area, and the glucose sensing properties of cuprous oxides are investigated and spotted that, 600 °C is an effective annealing temperature. Then, we developed an electrochemical biosensor through the economic SPCE modification method. As a result, the modified electrode showed exceptional electrocatalytic ability towards glucose and the anodic peak current is correlated with the concentrations of glucose. It obtained more extensive working range between 31 nM and 1423 μM and with very low detection limit and appreciable sensitivity. This method is successfully applied to the recognition of glucose level in the samples of human blood serum and whole blood.