Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels

Sanjay Kumar Gupta; Faiz Ahmad Ansari; Amritanshu Shriwastav; Narendra Kumar Sahoo; Ismail Rawat; Faizal Bux

doi:10.1016/j.jclepro.2015.12.040

Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels

Sanjay Kumar Gupta
, Faiz Ahmad Ansari
, Amritanshu Shriwastav
, Narendra Kumar Sahoo
, Ismail Rawat
, Faizal Bux^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

176 Scopus citations

Abstract

Microalgal treatment of raw sewage presents many complexities, mainly resulting from the inability of the algal species to sustain increased physiological stresses due to variable nutrient levels and high concentrations of organics. Chlorella sorokiniana and Scenedesmus obliquus have been identified to tolerate higher amounts of organic loading and physiological stresses. Nutrient removal, pathogen removal, and lipid accumulation with secondary or tertiary effluents have been demonstrated independently for these organisms. However, their potentials for accomplishing these objectives simultaneously with raw sewage have not been investigated. This study presents comprehensive investigations of applicability of C. sorokiniana and S. obliquus to wastewater treatment without the requirement for any additional treatment. S. obliquus showed greater potential for removing organic carbon (76.13 ± 1.59% COD removal), nutrients (98.54 ± 3.30% N-removal, 97.99 ± 3.59% P-removal) and comparable pathogens removal (99.93 ± 0.12% total coliforms removal, 100% faecal coliform removal) in comparison to C. sorokiniana (69.38 ± 1.81% COD removal, 86.93 ± 3.49% N-removal, 68.24 ± 11.69% P-removal, 99.78 ± 0.12% total coliforms removal, 100% faecal coliform removal) with 15 days of cultivation with filtered raw sewage, but also encountered increased levels of stress (F_v/F_m of 0.48 ± 0.03) which accounted for increased lipid accumulation in the cells (23.26 ± 3.95% w/w) but might also affect their biomass productivity and treatment potential in longer applications. In comparison, C. sorokiniana demonstrated better adaptability to physiological stresses (F_v/F_m of 0.53 ± 0.01) and may be suitable for achieving comprehensive treatment and sufficient lipid accumulation (22.74 ± 3.11% w/w) without compromising these potentials during prolonged applications. These results highlight the importance of selecting algal species with better stress resistance to extend their applicability for comprehensive wastewater treatment and lipid production.

Original language	English
Pages (from-to)	255-264
Number of pages	10
Journal	Journal of Cleaner Production
Volume	115
DOIs	https://doi.org/10.1016/j.jclepro.2015.12.040
State	Published - 1 Mar 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

Chlorella sorokiniana
Comprehensive wastewater treatment
Lipid production
Pathogen removal
Scenedesmus obliquus

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment
Building and Construction
General Environmental Science
Strategy and Management
Industrial and Manufacturing Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jclepro.2015.12.040

Cite this

@article{9afa10ead2b24a22ba41df7eeeb25d59,

title = "Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels",

abstract = "Microalgal treatment of raw sewage presents many complexities, mainly resulting from the inability of the algal species to sustain increased physiological stresses due to variable nutrient levels and high concentrations of organics. Chlorella sorokiniana and Scenedesmus obliquus have been identified to tolerate higher amounts of organic loading and physiological stresses. Nutrient removal, pathogen removal, and lipid accumulation with secondary or tertiary effluents have been demonstrated independently for these organisms. However, their potentials for accomplishing these objectives simultaneously with raw sewage have not been investigated. This study presents comprehensive investigations of applicability of C. sorokiniana and S. obliquus to wastewater treatment without the requirement for any additional treatment. S. obliquus showed greater potential for removing organic carbon (76.13 ± 1.59\% COD removal), nutrients (98.54 ± 3.30\% N-removal, 97.99 ± 3.59\% P-removal) and comparable pathogens removal (99.93 ± 0.12\% total coliforms removal, 100\% faecal coliform removal) in comparison to C. sorokiniana (69.38 ± 1.81\% COD removal, 86.93 ± 3.49\% N-removal, 68.24 ± 11.69\% P-removal, 99.78 ± 0.12\% total coliforms removal, 100\% faecal coliform removal) with 15 days of cultivation with filtered raw sewage, but also encountered increased levels of stress (Fv/Fm of 0.48 ± 0.03) which accounted for increased lipid accumulation in the cells (23.26 ± 3.95\% w/w) but might also affect their biomass productivity and treatment potential in longer applications. In comparison, C. sorokiniana demonstrated better adaptability to physiological stresses (Fv/Fm of 0.53 ± 0.01) and may be suitable for achieving comprehensive treatment and sufficient lipid accumulation (22.74 ± 3.11\% w/w) without compromising these potentials during prolonged applications. These results highlight the importance of selecting algal species with better stress resistance to extend their applicability for comprehensive wastewater treatment and lipid production.",

keywords = "Chlorella sorokiniana, Comprehensive wastewater treatment, Lipid production, Pathogen removal, Scenedesmus obliquus",

author = "Gupta, \{Sanjay Kumar\} and Ansari, \{Faiz Ahmad\} and Amritanshu Shriwastav and Sahoo, \{Narendra Kumar\} and Ismail Rawat and Faizal Bux",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

year = "2016",

month = mar,

day = "1",

doi = "10.1016/j.jclepro.2015.12.040",

language = "English",

volume = "115",

pages = "255--264",

journal = "Journal of Cleaner Production",

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TY - JOUR

T1 - Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels

AU - Gupta, Sanjay Kumar

AU - Ansari, Faiz Ahmad

AU - Shriwastav, Amritanshu

AU - Sahoo, Narendra Kumar

AU - Rawat, Ismail

AU - Bux, Faizal

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Microalgal treatment of raw sewage presents many complexities, mainly resulting from the inability of the algal species to sustain increased physiological stresses due to variable nutrient levels and high concentrations of organics. Chlorella sorokiniana and Scenedesmus obliquus have been identified to tolerate higher amounts of organic loading and physiological stresses. Nutrient removal, pathogen removal, and lipid accumulation with secondary or tertiary effluents have been demonstrated independently for these organisms. However, their potentials for accomplishing these objectives simultaneously with raw sewage have not been investigated. This study presents comprehensive investigations of applicability of C. sorokiniana and S. obliquus to wastewater treatment without the requirement for any additional treatment. S. obliquus showed greater potential for removing organic carbon (76.13 ± 1.59% COD removal), nutrients (98.54 ± 3.30% N-removal, 97.99 ± 3.59% P-removal) and comparable pathogens removal (99.93 ± 0.12% total coliforms removal, 100% faecal coliform removal) in comparison to C. sorokiniana (69.38 ± 1.81% COD removal, 86.93 ± 3.49% N-removal, 68.24 ± 11.69% P-removal, 99.78 ± 0.12% total coliforms removal, 100% faecal coliform removal) with 15 days of cultivation with filtered raw sewage, but also encountered increased levels of stress (Fv/Fm of 0.48 ± 0.03) which accounted for increased lipid accumulation in the cells (23.26 ± 3.95% w/w) but might also affect their biomass productivity and treatment potential in longer applications. In comparison, C. sorokiniana demonstrated better adaptability to physiological stresses (Fv/Fm of 0.53 ± 0.01) and may be suitable for achieving comprehensive treatment and sufficient lipid accumulation (22.74 ± 3.11% w/w) without compromising these potentials during prolonged applications. These results highlight the importance of selecting algal species with better stress resistance to extend their applicability for comprehensive wastewater treatment and lipid production.

AB - Microalgal treatment of raw sewage presents many complexities, mainly resulting from the inability of the algal species to sustain increased physiological stresses due to variable nutrient levels and high concentrations of organics. Chlorella sorokiniana and Scenedesmus obliquus have been identified to tolerate higher amounts of organic loading and physiological stresses. Nutrient removal, pathogen removal, and lipid accumulation with secondary or tertiary effluents have been demonstrated independently for these organisms. However, their potentials for accomplishing these objectives simultaneously with raw sewage have not been investigated. This study presents comprehensive investigations of applicability of C. sorokiniana and S. obliquus to wastewater treatment without the requirement for any additional treatment. S. obliquus showed greater potential for removing organic carbon (76.13 ± 1.59% COD removal), nutrients (98.54 ± 3.30% N-removal, 97.99 ± 3.59% P-removal) and comparable pathogens removal (99.93 ± 0.12% total coliforms removal, 100% faecal coliform removal) in comparison to C. sorokiniana (69.38 ± 1.81% COD removal, 86.93 ± 3.49% N-removal, 68.24 ± 11.69% P-removal, 99.78 ± 0.12% total coliforms removal, 100% faecal coliform removal) with 15 days of cultivation with filtered raw sewage, but also encountered increased levels of stress (Fv/Fm of 0.48 ± 0.03) which accounted for increased lipid accumulation in the cells (23.26 ± 3.95% w/w) but might also affect their biomass productivity and treatment potential in longer applications. In comparison, C. sorokiniana demonstrated better adaptability to physiological stresses (Fv/Fm of 0.53 ± 0.01) and may be suitable for achieving comprehensive treatment and sufficient lipid accumulation (22.74 ± 3.11% w/w) without compromising these potentials during prolonged applications. These results highlight the importance of selecting algal species with better stress resistance to extend their applicability for comprehensive wastewater treatment and lipid production.

KW - Chlorella sorokiniana

KW - Comprehensive wastewater treatment

KW - Lipid production

KW - Pathogen removal

KW - Scenedesmus obliquus

UR - https://www.scopus.com/pages/publications/85002342346

U2 - 10.1016/j.jclepro.2015.12.040

DO - 10.1016/j.jclepro.2015.12.040

M3 - Article

AN - SCOPUS:85002342346

SN - 0959-6526

VL - 115

SP - 255

EP - 264

JO - Journal of Cleaner Production

JF - Journal of Cleaner Production

ER -

Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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