DNA barcoding identifies brackish water fishes from Nallavadu Lagoon, Puducherry, India

V. Sachithanandam; P. M. Mohan; N. Muruganandam; R. Sivasankar; P. Arunkumar; T. Mageswaran; R. Sridhar

doi:10.1007/978-3-319-41840-7_16

DNA barcoding identifies brackish water fishes from Nallavadu Lagoon, Puducherry, India

V. Sachithanandam^*
, P. M. Mohan
, N. Muruganandam
, R. Sivasankar
, P. Arunkumar
, T. Mageswaran
, R. Sridhar

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

Animal DNA barcoding has been advanced as a promising tool to aid species identification and discovery through the use of short, standardized mitochondrial gene COI targets in spite of extensive morphological taxonomic studies, for a variety of reasons the identification of fishes can be problematic, time consuming, even for experts. To overcome in this content though DNA barcoding is proving to be a useful tool. The present study use DNA barcoding to genetically identify specimen were collected from Nallavadu Lagoon ecosystem in Puducherry and morphologically identified. The COI was amplified and bi-directionally sequenced from 45 specimens (5 specimens/species) belonging to 9 species, five families. All studied specimens sequence were interrogated using analytical tools developed by Barcode of Life Data System (BOLD). All specimen exhibited discrete clusters of closely related haplogroups in NJ tree, which permitted the discrimination of 96-100 % genetic similarity of all the studied species. These studies represent a significant contribution to the worldwide barcode database from Indian continental regional and demonstrated the utility of COI gene sequences for specific regional species identification.

Original language	English
Title of host publication	DNA Barcoding in Marine Perspectives
Subtitle of host publication	Assessment and Conservation of Biodiversity
Publisher	Springer International Publishing
Pages	271-283
Number of pages	13
ISBN (Electronic)	9783319418407
ISBN (Print)	9783319418384
DOIs	https://doi.org/10.1007/978-3-319-41840-7_16
State	Published - 1 Jan 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© Springer International Publishing Switzerland 2016.

Keywords

Brackish water
DNA barcoding
Nallavadu lagoon
Taxonomy

ASJC Scopus subject areas

General Agricultural and Biological Sciences
General Environmental Science
General Biochemistry, Genetics and Molecular Biology
General Medicine

Access to Document

10.1007/978-3-319-41840-7_16

Cite this

Sachithanandam, V., Mohan, P. M., Muruganandam, N., Sivasankar, R., Arunkumar, P., Mageswaran, T., & Sridhar, R. (2016). DNA barcoding identifies brackish water fishes from Nallavadu Lagoon, Puducherry, India. In DNA Barcoding in Marine Perspectives: Assessment and Conservation of Biodiversity (pp. 271-283). Springer International Publishing. https://doi.org/10.1007/978-3-319-41840-7_16

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abstract = "Animal DNA barcoding has been advanced as a promising tool to aid species identification and discovery through the use of short, standardized mitochondrial gene COI targets in spite of extensive morphological taxonomic studies, for a variety of reasons the identification of fishes can be problematic, time consuming, even for experts. To overcome in this content though DNA barcoding is proving to be a useful tool. The present study use DNA barcoding to genetically identify specimen were collected from Nallavadu Lagoon ecosystem in Puducherry and morphologically identified. The COI was amplified and bi-directionally sequenced from 45 specimens (5 specimens/species) belonging to 9 species, five families. All studied specimens sequence were interrogated using analytical tools developed by Barcode of Life Data System (BOLD). All specimen exhibited discrete clusters of closely related haplogroups in NJ tree, which permitted the discrimination of 96-100 \% genetic similarity of all the studied species. These studies represent a significant contribution to the worldwide barcode database from Indian continental regional and demonstrated the utility of COI gene sequences for specific regional species identification.",

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Sachithanandam, V, Mohan, PM, Muruganandam, N, Sivasankar, R, Arunkumar, P, Mageswaran, T & Sridhar, R 2016, DNA barcoding identifies brackish water fishes from Nallavadu Lagoon, Puducherry, India. in DNA Barcoding in Marine Perspectives: Assessment and Conservation of Biodiversity. Springer International Publishing, pp. 271-283. https://doi.org/10.1007/978-3-319-41840-7_16

DNA barcoding identifies brackish water fishes from Nallavadu Lagoon, Puducherry, India. / Sachithanandam, V.; Mohan, P. M.; Muruganandam, N. et al.
DNA Barcoding in Marine Perspectives: Assessment and Conservation of Biodiversity. Springer International Publishing, 2016. p. 271-283.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AU - Muruganandam, N.

AU - Sivasankar, R.

AU - Arunkumar, P.

AU - Mageswaran, T.

AU - Sridhar, R.

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DNA barcoding identifies brackish water fishes from Nallavadu Lagoon, Puducherry, India

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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