Development and validation of spectrophotometric method for the determination of nimesulide in bulk and tablet dosage forms by biuret reagent method

Muhammad Khalid Bhatti; Muhammad Munawar Hayat; Rubia Nasir; Faiz Ul Hassan Nasim; Muhammad Ashraf; Bushra Hussain

Development and validation of spectrophotometric method for the determination of nimesulide in bulk and tablet dosage forms by biuret reagent method

Muhammad Khalid Bhatti, Muhammad Munawar Hayat, Rubia Nasir, Faiz Ul Hassan Nasim, Muhammad Ashraf^*, Bushra Hussain

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The purpose of the study was to develop a method for the determination of Nimesulide using visible spectrophotometry. It is based on the reaction of Nimesulide with biuret reagent, which forms a greenish coloured complex. The formed complex exhibited maximum absorption at 400 nm. The limit of detection of Nimesulide was 2.5 μg mL -1 and the limit of quantification was 5.0 μg mL- 1. The developed method has acceptable accuracy (97-101%) and correlation coefficient (< 3%). The method was successfully applied on three different tablet dosage brands available in Pakistani market and statistical data shows that results are reliable and reproducible.

Original language	English
Pages (from-to)	713-716
Number of pages	4
Journal	Journal of the Chemical Society of Pakistan
Volume	34
Issue number	3
State	Published - Jun 2012
Externally published	Yes

Keywords

Biuret reagent
Cycloogygenase
Nimesulide
Spectrophotometry
Tablet dosage form

ASJC Scopus subject areas

General Chemistry

Cite this

@article{a34f9a149aa048fe94f5d0b33ab19187,

title = "Development and validation of spectrophotometric method for the determination of nimesulide in bulk and tablet dosage forms by biuret reagent method",

abstract = "The purpose of the study was to develop a method for the determination of Nimesulide using visible spectrophotometry. It is based on the reaction of Nimesulide with biuret reagent, which forms a greenish coloured complex. The formed complex exhibited maximum absorption at 400 nm. The limit of detection of Nimesulide was 2.5 μg mL -1 and the limit of quantification was 5.0 μg mL- 1. The developed method has acceptable accuracy (97-101\%) and correlation coefficient (< 3\%). The method was successfully applied on three different tablet dosage brands available in Pakistani market and statistical data shows that results are reliable and reproducible.",

keywords = "Biuret reagent, Cycloogygenase, Nimesulide, Spectrophotometry, Tablet dosage form",

author = "Bhatti, \{Muhammad Khalid\} and Hayat, \{Muhammad Munawar\} and Rubia Nasir and Nasim, \{Faiz Ul Hassan\} and Muhammad Ashraf and Bushra Hussain",

year = "2012",

month = jun,

language = "English",

volume = "34",

pages = "713--716",

journal = "Journal of the Chemical Society of Pakistan",

issn = "0253-5106",

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}

Development and validation of spectrophotometric method for the determination of nimesulide in bulk and tablet dosage forms by biuret reagent method. / Bhatti, Muhammad Khalid; Hayat, Muhammad Munawar; Nasir, Rubia et al.
In: Journal of the Chemical Society of Pakistan, Vol. 34, No. 3, 06.2012, p. 713-716.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development and validation of spectrophotometric method for the determination of nimesulide in bulk and tablet dosage forms by biuret reagent method

AU - Bhatti, Muhammad Khalid

AU - Hayat, Muhammad Munawar

AU - Nasir, Rubia

AU - Nasim, Faiz Ul Hassan

AU - Ashraf, Muhammad

AU - Hussain, Bushra

PY - 2012/6

Y1 - 2012/6

N2 - The purpose of the study was to develop a method for the determination of Nimesulide using visible spectrophotometry. It is based on the reaction of Nimesulide with biuret reagent, which forms a greenish coloured complex. The formed complex exhibited maximum absorption at 400 nm. The limit of detection of Nimesulide was 2.5 μg mL -1 and the limit of quantification was 5.0 μg mL- 1. The developed method has acceptable accuracy (97-101%) and correlation coefficient (< 3%). The method was successfully applied on three different tablet dosage brands available in Pakistani market and statistical data shows that results are reliable and reproducible.

AB - The purpose of the study was to develop a method for the determination of Nimesulide using visible spectrophotometry. It is based on the reaction of Nimesulide with biuret reagent, which forms a greenish coloured complex. The formed complex exhibited maximum absorption at 400 nm. The limit of detection of Nimesulide was 2.5 μg mL -1 and the limit of quantification was 5.0 μg mL- 1. The developed method has acceptable accuracy (97-101%) and correlation coefficient (< 3%). The method was successfully applied on three different tablet dosage brands available in Pakistani market and statistical data shows that results are reliable and reproducible.

KW - Biuret reagent

KW - Cycloogygenase

KW - Nimesulide

KW - Spectrophotometry

KW - Tablet dosage form

UR - https://www.scopus.com/pages/publications/84862195367

M3 - Article

AN - SCOPUS:84862195367

SN - 0253-5106

VL - 34

SP - 713

EP - 716

JO - Journal of the Chemical Society of Pakistan

JF - Journal of the Chemical Society of Pakistan

IS - 3

ER -

Development and validation of spectrophotometric method for the determination of nimesulide in bulk and tablet dosage forms by biuret reagent method

Abstract

Keywords

ASJC Scopus subject areas

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