Development and validation of a spectrophotometric method for the determination of entecavir in pure and tablet dosage form

Hafiz Muhammad Nauman Shabbir; Muhammad Munawar Hayat; Muhammad Ashraf; Faiz Ul Hassan Nasim; Irshad Ahmad; Muhammad Uzair; Naheed Haque

Development and validation of a spectrophotometric method for the determination of entecavir in pure and tablet dosage form

Hafiz Muhammad Nauman Shabbir
, Muhammad Munawar Hayat
, Muhammad Ashraf^*
, Faiz Ul Hassan Nasim
, Irshad Ahmad
, Muhammad Uzair
, Naheed Haque

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A direct spectrophotometric method for the determination of Entecavir is developed. Entecavir is a guanine analogue that is used in the treatment of hepatitis B. The absorbance of the pure drug was measured at wavelength 253 nm. The method was found linear from 2.5-40 μg mL^-1 (r², 0.999). The RSD was 1.1% and accuracy was 99%. The validation results and statistical data demonstrate that the method is reliable, simple, cost effective and reproducible and has an importance in quality assurance of Entecavir analysis.

Original language	English
Pages (from-to)	1605-1608
Number of pages	4
Journal	Journal of the Chemical Society of Pakistan
Volume	34
Issue number	6
State	Published - Dec 2012
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Entecavir
Spectrophotometer
Tablet dosage form

ASJC Scopus subject areas

General Chemistry

Cite this

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title = "Development and validation of a spectrophotometric method for the determination of entecavir in pure and tablet dosage form",

abstract = "A direct spectrophotometric method for the determination of Entecavir is developed. Entecavir is a guanine analogue that is used in the treatment of hepatitis B. The absorbance of the pure drug was measured at wavelength 253 nm. The method was found linear from 2.5-40 μg mL-1 (r2, 0.999). The RSD was 1.1\% and accuracy was 99\%. The validation results and statistical data demonstrate that the method is reliable, simple, cost effective and reproducible and has an importance in quality assurance of Entecavir analysis.",

keywords = "Entecavir, Spectrophotometer, Tablet dosage form",

author = "Shabbir, \{Hafiz Muhammad Nauman\} and Hayat, \{Muhammad Munawar\} and Muhammad Ashraf and Nasim, \{Faiz Ul Hassan\} and Irshad Ahmad and Muhammad Uzair and Naheed Haque",

year = "2012",

month = dec,

language = "English",

volume = "34",

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journal = "Journal of the Chemical Society of Pakistan",

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TY - JOUR

T1 - Development and validation of a spectrophotometric method for the determination of entecavir in pure and tablet dosage form

AU - Shabbir, Hafiz Muhammad Nauman

AU - Hayat, Muhammad Munawar

AU - Ashraf, Muhammad

AU - Nasim, Faiz Ul Hassan

AU - Ahmad, Irshad

AU - Uzair, Muhammad

AU - Haque, Naheed

PY - 2012/12

Y1 - 2012/12

N2 - A direct spectrophotometric method for the determination of Entecavir is developed. Entecavir is a guanine analogue that is used in the treatment of hepatitis B. The absorbance of the pure drug was measured at wavelength 253 nm. The method was found linear from 2.5-40 μg mL-1 (r2, 0.999). The RSD was 1.1% and accuracy was 99%. The validation results and statistical data demonstrate that the method is reliable, simple, cost effective and reproducible and has an importance in quality assurance of Entecavir analysis.

AB - A direct spectrophotometric method for the determination of Entecavir is developed. Entecavir is a guanine analogue that is used in the treatment of hepatitis B. The absorbance of the pure drug was measured at wavelength 253 nm. The method was found linear from 2.5-40 μg mL-1 (r2, 0.999). The RSD was 1.1% and accuracy was 99%. The validation results and statistical data demonstrate that the method is reliable, simple, cost effective and reproducible and has an importance in quality assurance of Entecavir analysis.

KW - Entecavir

KW - Spectrophotometer

KW - Tablet dosage form

UR - https://www.scopus.com/pages/publications/84874467812

M3 - Article

AN - SCOPUS:84874467812

SN - 0253-5106

VL - 34

SP - 1605

EP - 1608

JO - Journal of the Chemical Society of Pakistan

JF - Journal of the Chemical Society of Pakistan

IS - 6

ER -

Development and validation of a spectrophotometric method for the determination of entecavir in pure and tablet dosage form

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

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Cite this