Design and simulation of microchannel for DNA amplification

Muhammad Ahsan Saeed; Shafaat Ahmed Bazaz; Nisar Ahmed; Mujahid Naseem; Azizur Rehman

doi:10.1109/INMIC.2016.7840145

Design and simulation of microchannel for DNA amplification

Muhammad Ahsan Saeed
, Shafaat Ahmed Bazaz
, Nisar Ahmed
, Mujahid Naseem
, Azizur Rehman

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

Microfluidic technology has been playing a critical role in biochemical assays and molecular diagnosis since past few decades. One of its applications is polymerase chain reaction which requires fast and repetitive thermal cycling to amplify small amount of DNA. In this paper, microchannel is designed and CFD simulations are performed in ANSYS-CFX to obtain optimum velocity for efficient DNA amplification. The serpentine type channel of polycarbonate base covered with acetate layer is used. Two heaters of constant temperature are attached on the upper surface of the micro channels. Water is used as a carrier fluid and various parameters which may affect DNA amplification efficiency like flow rate, thermal mass, residence time, pumping power and heater powers are investigated. For related channel dimensions, optimum velocity of 4 mm/s and pumping power of 0.09 μW are obtained. Moreover, the impact of non-convection and natural convection on residence time is also analyzed numerically to optimize heater power.

Original language	English
Title of host publication	Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781509043002
DOIs	https://doi.org/10.1109/INMIC.2016.7840145
State	Published - 2 Feb 2017
Externally published	Yes

Publication series

Name	Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016

Bibliographical note

Publisher Copyright:
© 2016 IEEE.

Keywords

CFX
DNA amplification
MEMS
microfluidics
polymerase chain reaction

ASJC Scopus subject areas

Artificial Intelligence
Computer Networks and Communications
Instrumentation
Hardware and Architecture
Software
Energy Engineering and Power Technology
Control and Systems Engineering
Control and Optimization

Access to Document

10.1109/INMIC.2016.7840145

Cite this

Saeed, M. A., Bazaz, S. A., Ahmed, N., Naseem, M., & Rehman, A. (2017). Design and simulation of microchannel for DNA amplification. In Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016 Article 7840145 (Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/INMIC.2016.7840145

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title = "Design and simulation of microchannel for DNA amplification",

abstract = "Microfluidic technology has been playing a critical role in biochemical assays and molecular diagnosis since past few decades. One of its applications is polymerase chain reaction which requires fast and repetitive thermal cycling to amplify small amount of DNA. In this paper, microchannel is designed and CFD simulations are performed in ANSYS-CFX to obtain optimum velocity for efficient DNA amplification. The serpentine type channel of polycarbonate base covered with acetate layer is used. Two heaters of constant temperature are attached on the upper surface of the micro channels. Water is used as a carrier fluid and various parameters which may affect DNA amplification efficiency like flow rate, thermal mass, residence time, pumping power and heater powers are investigated. For related channel dimensions, optimum velocity of 4 mm/s and pumping power of 0.09 μW are obtained. Moreover, the impact of non-convection and natural convection on residence time is also analyzed numerically to optimize heater power.",

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Saeed, MA, Bazaz, SA, Ahmed, N, Naseem, M & Rehman, A 2017, Design and simulation of microchannel for DNA amplification. in Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016., 7840145, Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016, Institute of Electrical and Electronics Engineers Inc. https://doi.org/10.1109/INMIC.2016.7840145

Design and simulation of microchannel for DNA amplification. / Saeed, Muhammad Ahsan; Bazaz, Shafaat Ahmed; Ahmed, Nisar et al.
Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016. Institute of Electrical and Electronics Engineers Inc., 2017. 7840145 (Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Design and simulation of microchannel for DNA amplification

AU - Saeed, Muhammad Ahsan

AU - Bazaz, Shafaat Ahmed

AU - Ahmed, Nisar

AU - Naseem, Mujahid

AU - Rehman, Azizur

PY - 2017/2/2

Y1 - 2017/2/2

N2 - Microfluidic technology has been playing a critical role in biochemical assays and molecular diagnosis since past few decades. One of its applications is polymerase chain reaction which requires fast and repetitive thermal cycling to amplify small amount of DNA. In this paper, microchannel is designed and CFD simulations are performed in ANSYS-CFX to obtain optimum velocity for efficient DNA amplification. The serpentine type channel of polycarbonate base covered with acetate layer is used. Two heaters of constant temperature are attached on the upper surface of the micro channels. Water is used as a carrier fluid and various parameters which may affect DNA amplification efficiency like flow rate, thermal mass, residence time, pumping power and heater powers are investigated. For related channel dimensions, optimum velocity of 4 mm/s and pumping power of 0.09 μW are obtained. Moreover, the impact of non-convection and natural convection on residence time is also analyzed numerically to optimize heater power.

AB - Microfluidic technology has been playing a critical role in biochemical assays and molecular diagnosis since past few decades. One of its applications is polymerase chain reaction which requires fast and repetitive thermal cycling to amplify small amount of DNA. In this paper, microchannel is designed and CFD simulations are performed in ANSYS-CFX to obtain optimum velocity for efficient DNA amplification. The serpentine type channel of polycarbonate base covered with acetate layer is used. Two heaters of constant temperature are attached on the upper surface of the micro channels. Water is used as a carrier fluid and various parameters which may affect DNA amplification efficiency like flow rate, thermal mass, residence time, pumping power and heater powers are investigated. For related channel dimensions, optimum velocity of 4 mm/s and pumping power of 0.09 μW are obtained. Moreover, the impact of non-convection and natural convection on residence time is also analyzed numerically to optimize heater power.

KW - CFX

KW - DNA amplification

KW - MEMS

KW - microfluidics

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T3 - Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016

BT - Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016

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Saeed MA, Bazaz SA, Ahmed N, Naseem M, Rehman A. Design and simulation of microchannel for DNA amplification. In Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016. Institute of Electrical and Electronics Engineers Inc. 2017. 7840145. (Proceedings of the 2016 19th International Multi-Topic Conference, INMIC 2016). doi: 10.1109/INMIC.2016.7840145

Design and simulation of microchannel for DNA amplification

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this