Deciphering distinct root exudation, ionomics, and physio-biochemical attributes of Serratia marcescens CP-13 inoculated differentially Cd tolerant Zea mays cultivars

Cadmium (Cd) being a non-essential, mobile, and toxic heavy metal, negatively affects the plant growth and physiology. Current work investigated the impact of Serratia marcescens CP-13 inoculation on root organic acids and nutrient exudates of two maize cultivars varying in Cd tolerance under induced Cd toxicity. Seedlings of Cd-sensitive (Sahiwal-2002) and Cd-tolerant (MMRI-Yellow) cultivars were grown either inoculated or non-inoculated with CP-13 in Petri plates having various Cd stress levels (0, 6, 12, 18, 24, 30 μM). Seedlings were transferred to rhizoboxes for the collection of root exudates and analysis of physio-biochemical traits. Both maize cultivars exuded higher organic acids and nutrient exudates under non-inoculated conditions as compared to inoculated ones. Non-inoculated tolerant cultivar exhibited higher nutrient accumulation, biomass, antioxidants, total chlorophyll, Cd release meanwhile reduced Cd uptake, lipid peroxidation, exudation of organic acids, and nutrients than the sensitive one. However, under CP-13 inoculation, Cd sensitive cultivar exhibited less exudation of organic acids (citric acid, acetic acid, malic acid, glutamic acid, formic acid, succinic acid, and oxalic acid), nutrients mobilization (K, Na, Zn, Ca, and Mg), total chlorophyll, antioxidants (APX, SOD, POD), total soluble sugar, diminished MDA, and Cd uptake. The significant reduction in release of root exudates by both cultivars was likely due to the plant growth promoting traits of CP-13 which confer Cd tolerance. The maximum release of rhizospheric root exudates were documented at 30 μM applied Cd stress. Therefore, the Serratia sp. CP-13 was proposed as a potential inoculant for bioremediation of Cd together with maize cultivars.