CRISPR-based systems for sensitive and rapid on-site COVID-19 diagnostics

Jun Hui Soh; Enrique Balleza; Muhammad Nadjad Abdul Rahim; Hsi Min Chan; Siswand Mohd Ali; Jacqueline Kai Chin Chuah; Sherif Edris; Ahmed Atef; Ahmed Bahieldin; Jackie Y. Ying; Jamal S.M. Sabir

doi:10.1016/j.tibtech.2022.06.002

CRISPR-based systems for sensitive and rapid on-site COVID-19 diagnostics

Jun Hui Soh
, Enrique Balleza
, Muhammad Nadjad Abdul Rahim
, Hsi Min Chan
, Siswand Mohd Ali
, Jacqueline Kai Chin Chuah
, Sherif Edris
, Ahmed Atef
, Ahmed Bahieldin
, Jackie Y. Ying^*
, Jamal S.M. Sabir

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

52 Scopus citations

Abstract

The COVID-19 pandemic has strained healthcare systems. Sensitive, specific, and timely COVID-19 diagnosis is crucial for effective medical intervention and transmission control. RT-PCR is the most sensitive/specific, but requires costly equipment and trained personnel in centralized laboratories, which are inaccessible to resource-limited areas. Antigen rapid tests enable point-of-care (POC) detection but are significantly less sensitive/specific. CRISPR-Cas systems are compatible with isothermal amplification and dipstick readout, enabling sensitive/specific on-site testing. However, improvements in sensitivity and workflow complexity are needed to spur clinical adoption. We outline the mechanisms/strategies of major CRISPR-Cas systems, evaluate their on-site diagnostic capabilities, and discuss future research directions.

Original language	English
Pages (from-to)	1346-1360
Number of pages	15
Journal	Trends in Biotechnology
Volume	40
Issue number	11
DOIs	https://doi.org/10.1016/j.tibtech.2022.06.002
State	Published - Nov 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022 Elsevier Ltd

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

CRISPR-Cas
Cas12/13
RNA/DNA detection
diagnostics
infectious diseases
point-of-care

ASJC Scopus subject areas

Biotechnology
Bioengineering

Access to Document

10.1016/j.tibtech.2022.06.002

Cite this

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title = "CRISPR-based systems for sensitive and rapid on-site COVID-19 diagnostics",

abstract = "The COVID-19 pandemic has strained healthcare systems. Sensitive, specific, and timely COVID-19 diagnosis is crucial for effective medical intervention and transmission control. RT-PCR is the most sensitive/specific, but requires costly equipment and trained personnel in centralized laboratories, which are inaccessible to resource-limited areas. Antigen rapid tests enable point-of-care (POC) detection but are significantly less sensitive/specific. CRISPR-Cas systems are compatible with isothermal amplification and dipstick readout, enabling sensitive/specific on-site testing. However, improvements in sensitivity and workflow complexity are needed to spur clinical adoption. We outline the mechanisms/strategies of major CRISPR-Cas systems, evaluate their on-site diagnostic capabilities, and discuss future research directions.",

keywords = "CRISPR-Cas, Cas12/13, RNA/DNA detection, diagnostics, infectious diseases, point-of-care",

author = "Soh, \{Jun Hui\} and Enrique Balleza and \{Abdul Rahim\}, \{Muhammad Nadjad\} and Chan, \{Hsi Min\} and \{Mohd Ali\}, Siswand and Chuah, \{Jacqueline Kai Chin\} and Sherif Edris and Ahmed Atef and Ahmed Bahieldin and Ying, \{Jackie Y.\} and Sabir, \{Jamal S.M.\}",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

year = "2022",

month = nov,

doi = "10.1016/j.tibtech.2022.06.002",

language = "English",

volume = "40",

pages = "1346--1360",

journal = "Trends in Biotechnology",

issn = "0167-7799",

publisher = "Elsevier Ltd.",

number = "11",

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TY - JOUR

T1 - CRISPR-based systems for sensitive and rapid on-site COVID-19 diagnostics

AU - Soh, Jun Hui

AU - Balleza, Enrique

AU - Abdul Rahim, Muhammad Nadjad

AU - Chan, Hsi Min

AU - Mohd Ali, Siswand

AU - Chuah, Jacqueline Kai Chin

AU - Edris, Sherif

AU - Atef, Ahmed

AU - Bahieldin, Ahmed

AU - Ying, Jackie Y.

AU - Sabir, Jamal S.M.

PY - 2022/11

Y1 - 2022/11

N2 - The COVID-19 pandemic has strained healthcare systems. Sensitive, specific, and timely COVID-19 diagnosis is crucial for effective medical intervention and transmission control. RT-PCR is the most sensitive/specific, but requires costly equipment and trained personnel in centralized laboratories, which are inaccessible to resource-limited areas. Antigen rapid tests enable point-of-care (POC) detection but are significantly less sensitive/specific. CRISPR-Cas systems are compatible with isothermal amplification and dipstick readout, enabling sensitive/specific on-site testing. However, improvements in sensitivity and workflow complexity are needed to spur clinical adoption. We outline the mechanisms/strategies of major CRISPR-Cas systems, evaluate their on-site diagnostic capabilities, and discuss future research directions.

AB - The COVID-19 pandemic has strained healthcare systems. Sensitive, specific, and timely COVID-19 diagnosis is crucial for effective medical intervention and transmission control. RT-PCR is the most sensitive/specific, but requires costly equipment and trained personnel in centralized laboratories, which are inaccessible to resource-limited areas. Antigen rapid tests enable point-of-care (POC) detection but are significantly less sensitive/specific. CRISPR-Cas systems are compatible with isothermal amplification and dipstick readout, enabling sensitive/specific on-site testing. However, improvements in sensitivity and workflow complexity are needed to spur clinical adoption. We outline the mechanisms/strategies of major CRISPR-Cas systems, evaluate their on-site diagnostic capabilities, and discuss future research directions.

KW - CRISPR-Cas

KW - Cas12/13

KW - RNA/DNA detection

KW - diagnostics

KW - infectious diseases

KW - point-of-care

UR - https://www.scopus.com/pages/publications/85134821920

U2 - 10.1016/j.tibtech.2022.06.002

DO - 10.1016/j.tibtech.2022.06.002

M3 - Review article

C2 - 35871983

AN - SCOPUS:85134821920

SN - 0167-7799

VL - 40

SP - 1346

EP - 1360

JO - Trends in Biotechnology

JF - Trends in Biotechnology

IS - 11

ER -

CRISPR-based systems for sensitive and rapid on-site COVID-19 diagnostics

Abstract

Bibliographical note

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this