Column solid-phase extraction of sunset yellow and spectrophotometric determination of its use in powdered beverage and confectionery products

A new and simple solid-phase extraction method for the determination of trace levels of sunset yellow has been developed on Diaion HP 2MG column system. The adsorbed sunset yellow on the resin was eluted with 3mL of methanol and determined by UV-Vis spectrophotometry at 484nm. Various experimental parameters such as pH, amount of sunset yellow, flow rates of sample and eluent solutions, resin amount, sample volumes, etc. were investigated. The influences of some alkali, alkali earth and transition metals on the recoveries of sunset yellow were investigated. The recovery values for sunset yellow were higher than 95%. Preconcentration factor was found as 150. The detection limit (three sigma) of the reagent blank for sunset yellow was 5.2μgL ^-1. The relative standard deviations (RSD) of the procedure were found as 3.9%. The validation of the presented procedure was performed by the method of standard addition. The procedure was successfully applied to the determination of trace levels of sunset yellow in powdered beverage and confectionery products.