Clinical enzymes inhibitory activities, antioxidant potential and phytochemical profile of Vernonia oligocephala (DC.) Sch.Bip. ex Walp roots

  • Waqas Mahmood
  • , Hammad Saleem
  • , Wardah Shahid
  • , Irshad Ahmad
  • , Gokhan Zengin
  • , Mohamad Fawzi Mahomoodally*
  • , M. Ashraf
  • , Nafees Ahemad
  • *Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We investigated into the different solvent extracts (methanol, dichloromethane, ethyl acetate, n-hexane and n-butanol) of Vernonia oligocephala (DC.) Sch.Bip. ex Walp roots for their phytochemical composition, antioxidant and enzyme inhibition potential. Phytochemical analysis showed the presence of flavonoids, saponins, terpenoids, and phenolics as secondary metabolites. Methanol extract exhibited the highest phenolic (113.11 mg GAE/g) and flavonoid (97.35 mg QE/g) contents as well as ferric reducing antioxidant power (71.92 GAE/g). The ethyl acetate extract exhibited maximum DPPH (IC 50 ; 39.11 μg/mL), total antioxidant capacity (73.07 mg GAE/g) and urease inhibition (IC 50 ; 55.89 μg/mL). The n-hexane fraction was most active against cholinesterases. All extracts showed least activity against tyrosinase and lipoxygenase (except butanol fraction, IC 50 : 132.2 μg/mL). This study showed that V. oligocephala extracts can be regarded as perspective material for isolating bioactive molecules.

Original languageEnglish
Article number101039
JournalBiocatalysis and Agricultural Biotechnology
Volume18
DOIs
StatePublished - Mar 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

  • Antioxidant
  • Cholinesterases
  • Lipoxygenase
  • Phytochemical
  • Tyrosinase
  • Urease

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

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