Cell-adhesive thermogelling PNIPAAm/hyaluronic acid cell delivery hydrogels for potential application as minimally invasive retinal therapeutics

M. A.Jafar Mazumder; Scott D. Fitzpatrick; Ben Muirhead; Heather Sheardown

doi:10.1002/jbm.a.34021

Cell-adhesive thermogelling PNIPAAm/hyaluronic acid cell delivery hydrogels for potential application as minimally invasive retinal therapeutics

M. A.Jafar Mazumder, Scott D. Fitzpatrick, Ben Muirhead, Heather Sheardown^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Copolymers of N-isopropylacrylamide (NIPAAm) and acrylic acid N-hydroxysuccinimide (NAS) were synthesized via free radical polymerization and conjugated with amine-functionalized hyaluronic acid (HA) and cell adhesive RGDS peptides. These novel copolymers were designed to facilitate noninvasive delivery of a liquid suspension of cells into the delicate subretinal space for treatment of retinal degenerative diseases such as age-related macular degeneration (AMD) and diabetic retinopathy. The various synthesized copolymers all displayed subphysiological phase transition temperatures, thereby allowing temperature-induced scaffold formation and subsequent entrapment of transplanted cells within an adhesive support matrix. Successful grafting of HA and RGDS peptides were confirmed with Fourier Transform Infrared (FTIR) spectroscopy and quantified with ¹H Nuclear Magnetic Resonance (NMR) spectroscopy. All copolymers demonstrated excellent compatibility with retinal pigment epithelial (RPE) cells in culture and minimal host response was observed following subcutaneous implantation into hairless SKH1-E mice (strain code 447).

Original language	English
Pages (from-to)	1877-1887
Number of pages	11
Journal	Journal of Biomedical Materials Research - Part A
Volume	100 A
Issue number	7
DOIs	https://doi.org/10.1002/jbm.a.34021
State	Published - Jul 2012
Externally published	Yes

Keywords

cell delivery
hyaluronic acid
poly(N-isopropylacrylamide)
retina
thermally responsive

ASJC Scopus subject areas

Ceramics and Composites
Biomaterials
Biomedical Engineering
Metals and Alloys

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10.1002/jbm.a.34021

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@article{c5c3a8df7b1b40e483fddb23ce9d01ff,

title = "Cell-adhesive thermogelling PNIPAAm/hyaluronic acid cell delivery hydrogels for potential application as minimally invasive retinal therapeutics",

abstract = "Copolymers of N-isopropylacrylamide (NIPAAm) and acrylic acid N-hydroxysuccinimide (NAS) were synthesized via free radical polymerization and conjugated with amine-functionalized hyaluronic acid (HA) and cell adhesive RGDS peptides. These novel copolymers were designed to facilitate noninvasive delivery of a liquid suspension of cells into the delicate subretinal space for treatment of retinal degenerative diseases such as age-related macular degeneration (AMD) and diabetic retinopathy. The various synthesized copolymers all displayed subphysiological phase transition temperatures, thereby allowing temperature-induced scaffold formation and subsequent entrapment of transplanted cells within an adhesive support matrix. Successful grafting of HA and RGDS peptides were confirmed with Fourier Transform Infrared (FTIR) spectroscopy and quantified with 1H Nuclear Magnetic Resonance (NMR) spectroscopy. All copolymers demonstrated excellent compatibility with retinal pigment epithelial (RPE) cells in culture and minimal host response was observed following subcutaneous implantation into hairless SKH1-E mice (strain code 447).",

keywords = "cell delivery, hyaluronic acid, poly(N-isopropylacrylamide), retina, thermally responsive",

author = "Mazumder, \{M. A.Jafar\} and Fitzpatrick, \{Scott D.\} and Ben Muirhead and Heather Sheardown",

year = "2012",

month = jul,

doi = "10.1002/jbm.a.34021",

language = "English",

volume = "100 A",

pages = "1877--1887",

journal = "Journal of Biomedical Materials Research - Part A",

issn = "1549-3296",

publisher = "John Wiley \& Sons Inc.",

number = "7",

}

Cell-adhesive thermogelling PNIPAAm/hyaluronic acid cell delivery hydrogels for potential application as minimally invasive retinal therapeutics. / Mazumder, M. A.Jafar; Fitzpatrick, Scott D.; Muirhead, Ben et al.
In: Journal of Biomedical Materials Research - Part A, Vol. 100 A, No. 7, 07.2012, p. 1877-1887.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cell-adhesive thermogelling PNIPAAm/hyaluronic acid cell delivery hydrogels for potential application as minimally invasive retinal therapeutics

AU - Mazumder, M. A.Jafar

AU - Fitzpatrick, Scott D.

AU - Muirhead, Ben

AU - Sheardown, Heather

PY - 2012/7

Y1 - 2012/7

N2 - Copolymers of N-isopropylacrylamide (NIPAAm) and acrylic acid N-hydroxysuccinimide (NAS) were synthesized via free radical polymerization and conjugated with amine-functionalized hyaluronic acid (HA) and cell adhesive RGDS peptides. These novel copolymers were designed to facilitate noninvasive delivery of a liquid suspension of cells into the delicate subretinal space for treatment of retinal degenerative diseases such as age-related macular degeneration (AMD) and diabetic retinopathy. The various synthesized copolymers all displayed subphysiological phase transition temperatures, thereby allowing temperature-induced scaffold formation and subsequent entrapment of transplanted cells within an adhesive support matrix. Successful grafting of HA and RGDS peptides were confirmed with Fourier Transform Infrared (FTIR) spectroscopy and quantified with 1H Nuclear Magnetic Resonance (NMR) spectroscopy. All copolymers demonstrated excellent compatibility with retinal pigment epithelial (RPE) cells in culture and minimal host response was observed following subcutaneous implantation into hairless SKH1-E mice (strain code 447).

AB - Copolymers of N-isopropylacrylamide (NIPAAm) and acrylic acid N-hydroxysuccinimide (NAS) were synthesized via free radical polymerization and conjugated with amine-functionalized hyaluronic acid (HA) and cell adhesive RGDS peptides. These novel copolymers were designed to facilitate noninvasive delivery of a liquid suspension of cells into the delicate subretinal space for treatment of retinal degenerative diseases such as age-related macular degeneration (AMD) and diabetic retinopathy. The various synthesized copolymers all displayed subphysiological phase transition temperatures, thereby allowing temperature-induced scaffold formation and subsequent entrapment of transplanted cells within an adhesive support matrix. Successful grafting of HA and RGDS peptides were confirmed with Fourier Transform Infrared (FTIR) spectroscopy and quantified with 1H Nuclear Magnetic Resonance (NMR) spectroscopy. All copolymers demonstrated excellent compatibility with retinal pigment epithelial (RPE) cells in culture and minimal host response was observed following subcutaneous implantation into hairless SKH1-E mice (strain code 447).

KW - cell delivery

KW - hyaluronic acid

KW - poly(N-isopropylacrylamide)

KW - retina

KW - thermally responsive

UR - https://www.scopus.com/pages/publications/84861578755

U2 - 10.1002/jbm.a.34021

DO - 10.1002/jbm.a.34021

M3 - Article

C2 - 22492502

AN - SCOPUS:84861578755

SN - 1549-3296

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SP - 1877

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JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

IS - 7

ER -

Cell-adhesive thermogelling PNIPAAm/hyaluronic acid cell delivery hydrogels for potential application as minimally invasive retinal therapeutics

Abstract

Keywords

ASJC Scopus subject areas

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